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219215

Sigma-Aldrich

Leucocrystal Violet

Synonym(s):

4,4′,4′′-Methylidynetris(N,N-dimethylaniline), Leuco Crystal Violet

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About This Item

Linear Formula:
CH[C6H4N(CH3)2]3
CAS Number:
603-48-5
Molecular Weight:
373.53
EC Number:
210-043-9
MDL number:
MFCD00008314
UNSPSC Code:
12171500
PubChem Substance ID:
24853114
NACRES:
NA.47

form

powder

Quality Level

200

mp

175-177 °C (lit.)

λmax

260 nm

ε (extinction coefficient)

≥700 at 257-263 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

CN(C)c1ccc(cc1)C(c2ccc(cc2)N(C)C)c3ccc(cc3)N(C)C

InChI

1S/C25H31N3/c1-26(2)22-13-7-19(8-14-22)25(20-9-15-23(16-10-20)27(3)4)21-11-17-24(18-12-21)28(5)6/h7-18,25H,1-6H3

InChI key

OAZWDJGLIYNYMU-UHFFFAOYSA-N

Application

Leucocrystal Violet has been used to identify histamine-producing lactobacilli in cheese.

Biochem/physiol Actions

Leucocrystal Violet (LCV) is a cationic triarylmethane dye. LCV is a reduced form of gentian violet (crystal violet) and thus, it is colorless. It has an affinity for both cellulosic and proteinaceous materials. LCV and hydrogen peroxide react with the hemoglobin in blood and turn the blood impression to purple/violet color. Therefore, the dye is widely used to stain blood residue on both porous and non-porous materials.

Caution

Photo-ionization can cause color variations

related product

Product No.
Description
Pricing

Pictograms

Environment
GHS09

Signal Word

Warning

Hazard Statements

H410

Precautionary Statements

P273 - P391 - P501

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
MKBW1177V
MKBR8820V
MKBR8440V
S41106
S11859

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J E Roybal et al.
Journal - Association of Official Analytical Chemists, 73(6), 940-946 (1990-11-01)
A method is presented for determination of residues of gentian violet (GV), its demethylated metabolites (pentamethyl and tetramethyl), and leucogentian violet (LGV) in chicken tissue. The analytes are extracted from tissue with acetonitrile/buffer and partitioned into methylene chloride. Polar lipids
P G Schermerhorn et al.
Journal of AOAC International, 77(6), 1454-1460 (1994-11-01)
A laboratory trial was completed for a liquid chromatographic method that can quantitate leucogentian violet (LGV) in chicken fat at 10 ppb. With this method, LGV is isolated from the fat matrix by a series of liquid-liquid extractions. This trial
L G Rushing et al.
Journal of chromatography. B, Biomedical applications, 674(1), 125-131 (1995-12-01)
A sensitive analytical procedure for the determination of residues of leucogentian violet (LGV) and gentian violet (GV) in catfish tissue is presented. Frozen (-20 degrees C) catfish fillets were cut into chunks and then blended in a Waring blender. A
R T Wilson et al.
Journal of AOAC International, 77(5), 1137-1142 (1994-09-01)
A gas chromatographic/mass spectrometric (GC/MS) procedure for confirming the identity of leucogentian violet (LGV) in chicken fat was developed for regulatory application. The unused portion of the extract remaining from a determinative procedure was back-extracted into an organic phase, concentrated
Xueli Wu et al.
Journal of chromatography. A, 1172(2), 121-126 (2007-10-24)
A method has been developed for the simultaneous determination of malachite green, gentian violet and their leuco-metabolites in various aquatic products using isotope dilution liquid chromatography-linear ion trap mass spectrometry without post-column oxidation. Sample was extracted with McIlvaine buffer and
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