GC Analysis of Terpenes in Hops (Cascade Variety) on Equity-1 after Headspace SPME using 50/30 μm DVB/CAR/PDMS Fiber
Materials
SPME 파이버
SPME Fiber Assembly
50/30µm DVB/CAR/PDMS, StableFlex (1 cm), needle size 23 ga, Autosampler, pk of 3, plain gray hub부속물
Headspace vial, screw top, rounded bottom (vial only)
volume 10 mL, clear glass vial, thread for 18, O.D. × H 22.5 mm × 46 mm, pkg of 100 eaMagnetic Screw Cap for Headspace Vials, 18 mm thread
PTFE/silicone septum (white PTFE/blue silicone), septum thickness 1.5 mm, pkg of 100 ea분석 컬럼
표준 물질
C7 - C40 Saturated Alkanes Standard
certified reference material, 1000 μg/mL each component in hexaneCONDITIONS
sample/matrix
ground Cascade variety hop pellets
SPME fiber
Divinylbenzene/Carboxen/Polydimethylsiloxane (DVB/CAR/PDMS), 50/30 μm (57298-U)
extraction
20 min, headspace, 40 °C
desorption process
3 min, 270 °C
sample preparation
30 min equilibration at 40 °C prior to extraction
sample preparation
fiber postbake after extraction, 3 min at 270 °C
column
Equity-1, 60 m x 0.25 mm I.D., 0.25 μm (28047-U)
oven
60 °C (2 min), 5 °C/min to 275 °C (5 min)
inj. temp.
270 °C
detector
MSD
MSD interface
300 °C
scan range
full scan, m/z 50-500
carrier gas
helium, 1 mL/min constant flow
liner
0.75 mm ID, SPME
설명
분석 메모
Terpenes are contained in the essential oils derived from plants, and often impart characteristic aromas to the plant and/or oil. In this application, headspace-SPME combined with GC/MS was used to analyze some of the terpenes present in the Cascade variety of hops. Chromatographic separation was performed on an Equity-1 capillary GC column, and identification was done using retention indices and spectral library match. Analysis showed a characteristic terpene profile, with high levels of ß-myrcene, caryophyllene, and humulene present.
기타 정보
add 0.5 gm ground hops to a 10 mL headspace vial
법적 정보
null