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Merck
  • Flow-through enzyme immobilized amperometric detector for the rapid screening of acetylcholinesterase inhibitors by flow injection analysis.

Flow-through enzyme immobilized amperometric detector for the rapid screening of acetylcholinesterase inhibitors by flow injection analysis.

Journal of pharmaceutical and biomedical analysis (2014-12-03)
Marie Vandeput, Cobra Parsajoo, Jérôme Vanheuverzwijn, Stéphanie Patris, Yavuz Yardim, Alexandre le Jeune, Ahmad Sarakbi, Dominique Mertens, Jean-Michel Kauffmann
초록

A commercially available thin-layer flow-through amperometric detector, with the sensing block customized in an original design, was applied to the screening of drug compounds known as acetylcholinesterase (AChE) inhibitors. AChE from electric eel was covalently immobilized onto a cysteamine modified gold disk adjacent to a silver disk working electrode. On-line studies were performed by flow injection analysis (FIA) in PBS buffer pH 7.4. Seven commercially available AChE inhibitors used in the medical field, namely neostigmine, eserine, tacrine, donepezil, rivastigmine, pyridostigmine and galantamine as well as two natural compounds, quercetin and berberine, were investigated. The same trend of inhibitory potency as described in the literature was observed. Of particular interest and in addition to the determination of the IC50 values, this flow-through system allowed the study of both, the stability of the enzyme-inhibitor complex and the kinetic of the enzyme activity recovery.

MATERIALS
제품 번호
브랜드
제품 설명

Sigma-Aldrich
Pyridostigmine bromide
Sigma-Aldrich
Glutaraldehyde solution, 50% in H2O, suitable for photographic applications
Sigma-Aldrich
Galanthamine hydrobromide from Lycoris sp., ≥94% (HPLC)
Sigma-Aldrich
Glutaraldehyde solution, Grade I, 25% in H2O, specially purified for use as an electron microscopy fixative
Sigma-Aldrich
Glutaraldehyde solution, Grade I, 50% in H2O, specially purified for use as an electron microscopy fixative or other sophisticated use
Sigma-Aldrich
Glutaraldehyde solution, Grade I, 8% in H2O, specially purified for use as an electron microscopy fixative or other sophisticated use
Sigma-Aldrich
Glutaraldehyde solution, Grade I, 70% in H2O, specially purified for use as an electron microscopy fixative or other sophisticated use
Sigma-Aldrich
Glutaraldehyde solution, technical, ~50% in H2O (5.6 M)
Sigma-Aldrich
Neostigmine methyl sulfate
Sigma-Aldrich
9-Amino-1,2,3,4-tetrahydroacridine hydrochloride hydrate, ≥99%
Sigma-Aldrich
Glutaric dialdehyde solution, 50 wt. % in H2O, FCC
USP
Pyridostigmine bromide, United States Pharmacopeia (USP) Reference Standard
Neostigmine methyl sulfate, European Pharmacopoeia (EP) Reference Standard
Pyridostigmine bromide, European Pharmacopoeia (EP) Reference Standard
Sigma-Aldrich
Glutaraldehyde solution, Grade II, 25% in H2O
Sigma-Aldrich
9-Amino-1,2,3,4-tetrahydroacridine hydrochloride hydrate, ≥99%
Sigma-Aldrich
Donepezil hydrochloride, ≥98% (HPLC)
Sigma-Aldrich
Glutaraldehyde solution, 50 wt. % in H2O
Sigma-Aldrich
Berberine chloride form
Sigma-Aldrich
Berberine chloride hydrate, technical, ≥90% (AT)
Sigma-Aldrich
DL-Cysteine, technical grade
Sigma-Aldrich
Cysteamine hydrochloride, BioXtra
Sigma-Aldrich
Cysteamine hydrochloride, ≥97.0% (RT)
Sigma-Aldrich
Cysteamine hydrochloride, ≥98% (titration)
Galantamine hydrobromide, European Pharmacopoeia (EP) Reference Standard
USP
Galantamine hydrobromide, United States Pharmacopeia (USP) Reference Standard
Supelco
Berberine Chloride, Pharmaceutical Secondary Standard; Certified Reference Material
Quercetin dihydrate, primary reference standard
Quercetin dihydrate, European Pharmacopoeia (EP) Reference Standard