G006032

HPLC Analysis MAb Variants on Proteomix^® SCX-NP5, Fast Analysis

application for HPLC

속성

• 기술: HPLC: suitable
• 검사 파라미터: column: Proteomix SCX-NP5, 25 cm x 4.6 mm I.D., 5 μm particles (Z777164); mobile phase: [A] 2.4 mM Tris, 1.5 mM imidazole, 11.6 mM piperazine, pH 6.0; [B] A + 0.5 M NaCl, pH 10.5; gradient: top (0.8 mL/min): 5% B held for 5 min; to 29.5% B in 35 min; bottom (1.5 mL/min): 5% B held for 2.7 min; to 29.5% B in 18.6 min; flow rate: 0.8 mL/min or 1.5 mL/min; detector: UV 280 nm; injection: 20 μL; sample: MAb 321, 5 mg/mL
• 적합성: application for HPLC
• 응용 분야: life science and biopharma

설명

분석 메모

Due to the low pressure of Proteomix SCX, faster analysis and high resolution can be achieved with a higher flow rate.

법적 정보

Proteomix is a registered trademark of Sepax Technologies