G005861

HPLC Analysis of Fat-Soluble Vitamins on Ascentis^® Express C18, Methanol Mobile Phase

application for HPLC

속성

• 기술: HPLC: suitable
• 검사 파라미터: column: Ascentis Express C18, 15 cm x 4.6 mm I.D., 2.7 μm particles (53829-U); mobile phase: methanol; flow rate: 1.5 mL/min; pressure: 3249 psi (224 bar); column temp.: 30 °C; detector: UV, 280 nm; injection: 5 μL; sample: 0.5 mg/mL tocopherol acetate, alpha-tocopherol, delta-tocopherol and K1, 0.05 mg/mL D2, D3 and retinol acetate, 1.5 mg/mL retinol palmitate, all in ethanol.
• 적합성: application for HPLC
• 응용 분야: vitamins, nutraceuticals, and natural products

설명

분석 메모

Analysis was conducted using standard solution, detection via UV.

법적 정보

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

분석물

Retinyl acetate, analytical standard
δ-Tocopherol, analytical standard
Ergocalciferol (D2), analytical standard
Cholecalciferol (D3), analytical standard
(±)-α-Tocopherol, analytical standard
DL-alpha-Tocopherol acetate, analytical standard
Phylloquinone (K1), analytical standard
Retinol palmitate, analytical standard
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