G005675

HPLC Analysis of Benzodiazepines on Ascentis^® Express Phenyl-Hexyl with Acetonitrile Mobile Phase

application for HPLC

속성

• 기술: HPLC: suitable
• 검사 파라미터: column: Ascentis Express Phenyl-Hexyl, 5 cm x 4.6 mm I.D., 2.7 μm particles (53348-U); mobile phase: [A] water; [B] acetonitrile; (62:38, A:B); flow rate: 1.4 mL/min; pressure: 2600 psi (179 bar); column temp.: 35 °C; detector: UV, 250 nm; injection: 2 μL; sample: 0.1 g/mL of each in 20% methanol
• 적합성: application for HPLC
• 응용 분야: clinical; pharmaceutical (small molecule)

설명

분석 메모

The Phenyl-Hexyl phase of the Ascentis Express product line displays the best selectivity of this sample; selectivity is markedly affected by choice of organic modifier. The application also demonstrates feasibility of fast LC with Ascentis Express by virtue of low backpressure and high efficiency.

법적 정보

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

분석물

Oxazepam
Alprazolam
Clonazepam
Desmethyldiazepam
Diazepam