G005613

HPLC Analysis of Withania Extract on Ascentis^® Express F5

application for HPLC

속성

• 기술: HPLC: suitable
• 검사 파라미터: column: Ascentis Express F5, 10 cm x 2.1 mm I.D., 2.7 μm particles (53569-U); mobile phase: [A] water; [B] acetonitrile; gradient: 20 to 100% B in 5 min; held at 100% B for 0.5 min; flow rate: 0.3 mL/min; pressure: 2750 psi (190 bar); column temp.: 35 °C; detector: UV, 227 nm; injection: 5 μL; sample: 20 mg/mL in water:acetonitrile (50:50); sonicate for 15 minutes; filter 0.45 μm; dilute to water:acetonitrile (80:20)
• 적합성: application for HPLC
• 응용 분야: vitamins, nutraceuticals, and natural products

설명

분석 메모

This application demonstrates the suitability of Ascentis Express F5 for the efficient separation of withania extract.

법적 정보

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

분석물

Withanoside IV
Withanoside V
Withaferin A
12-Deoxywithastramonolide
Withanolide A
Withanone
Withanolide B