기술
HPLC: suitable
검사 파라미터
column: TSKgel CM-STAT, 10 cm x 4.6 mm I.D., 7 μm particles (821966)
mobile phase: [A] 20 mM MES buffer, pH 6.0; [B] 0.5 M NaCl in buffer A, pH 6.0
gradient: 0% B (0 min), 30% B (15 min), 100% B (15 min),
0% B (17 min), 10% B (17 min), 10% B (21 min)
flow rate: 1 mL/min
column temp.: ambient
detector: UV, 280 nm
injection: 20 μL
sample: monoclonal antibodies (mAb A through E)
적합성
application for HPLC
응용 분야
life science and biopharma
분석 메모
A TSKgel CM-STAT weak cation exchange (WCX) column was applied to separate charge variants of several monoclonal antibodies. The typical analysis time on conventional 25 cm long WCX columns of about eighty minutes could be significantly reduced when separation was performed on a 10 cm TSKgel CM-STAT column, filled with 7 μm particles. The analysis profiles for five antibodies show that high resolution analysis can be obtained in about 20 minutes analysis time.
문헌인용
From "Ion Exchange Chromatography for the Characterization of Biotherapeutics" in The Supelco Reporter, Vol. 29.3, page 20.
법적 정보
TSKgel is a registered trademark of Tosoh Corporation
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