G005374

HPLC Analysis of 25-Hydroxyvitamin D₃ and 25-Hydroxyvitamin D₂ on Ascentis^® Express F5

application for HPLC

속성

• 기술: HPLC: suitable
• 검사 파라미터: mobile phase: [A] 7 mM ammonium formate; [B] methanol; (25:75, A:B); flow rate: 0.4 mL/min; column temp.: 40 °C; detector: UV, 250 nm; ; ESI(+), TIC m/z 100 - 1000; injection: 1 μL; sample: 300 mg/L in 25:75, water:methanol; column: Ascentis Express F5, 10 cm x 2.1 mm I.D., 2.7 μm particles (53569-U)
• 적합성: application for HPLC
• 응용 분야: clinical; vitamins, nutraceuticals, and natural products

설명

법적 정보

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

분석물

25-Hydroxyvitamin D3 solution, 5 μg/mL in ethanol, ampule of 1 mL, certified reference material, Cerilliant^®
25-Hydroxyvitamin D2 solution, 50 μg/mL in ethanol, ampule of 1 mL, certified reference material, Cerilliant^®