813066

TSKgel^® Boronate-5PW (10 µm) HPLC Columns

L x I.D. 7.5 cm × 7.5 mm, m-aminophenyl boronate phase

• 동의어(들): TSKgel^® Boronate-5PW HPLC Column
• UNSPSC 코드: 41115700

속성

• 제품명: TSKgel^® Boronate-5PW HPLC Column, phase m-aminophenyl boronate, L × I.D. 7.5 cm × 7.5 mm, 10 μm particle size
• material: PEEK
• 제품 라인: TSKgel^®
• 분석물 화학적 분류: proteins
• 제조업체/상표: Tosoh Boronate-5PW, 13066
• 파라미터: ≤20% organic solvent; ≤3 M salt concentration; 1.2 mL/min flow rate; 30 bar max. pressure; ≥ 0 °C temperature
• 기술: HPLC: suitable
• 길이 × I.D.: 7.5 cm × 7.5 mm
• Matrix: polymer particle platform
• 기질 활성군: m-aminophenyl boronate phase
• 입자 크기: 10 μm
• 공극 크기: 1000 Å
• 작동 pH: 2-9
• 용량: 40 μmol/mL adsorption capacity (sorbitol)
• 호환성: mode of use affinity HPLC
• 분리 기술: affinity
• 저장 온도: 2-8°C

설명

일반 설명

High efficiency, resin-based TSKgel affinity columns (10 μm particles) separate or purify many enzymes and other proteins.

Coupling of m-aminophenyl boronate to TSKgel 5PW polymeric support results in a ligand capable of forming a tetrahedral boronate anion under alkaline pH conditions. This anionic structure can bind with 1,2 cis-diol groups such as those found in carbohydrates, carbohydrate containing compounds and catecholamines. Interaction between the boronate anion and the 1,2 cis-diol groups is enhanced in the presence of Mg2+ ions and is inhibited by amine-containing buffers. Adsorption onto the TSKgel Boronate-5PW matrix takes place in basic buffers such as HEPES and morpholine while desorption takes place in carbohydrate or amine-containing mobile phase like sorbitol or Tris.

Applications include: nucleic acids, catecholamines, nucleosides and nucleotides.

추천 제품

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

법적 정보

TSKgel is a registered trademark of Tosoh Corporation

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable