product name
TSKgel® Chelate-5PW Guardgel Kit, phase iminodiacetic acid, L × I.D. 2.5 cm × 6 mm, 20 μm particle size
material
PEEK
제품 라인
TSKgel®
제조업체/상표
Tosoh Chelate-5PW, 08647
라벨링 범위
20 μmol/mL resin ligand density
파라미터
≤20% organic solvent
≤3 M salt concentration
≤45 °C temperature
기술
LPLC: suitable
길이 × I.D.
2.5 cm × 6 mm
기질
polymer particle platform
기질 활성군
iminodiacetic acid phase
입자 크기
20 μm
작동 pH
2-12
호환성
mode of use affinity HPLC
분리 기술
affinity
일반 설명
High efficiency, resin-based TSKgel affinity columns (10 μm particles) separate or purify many enzymes and other proteins.
TSKgel Chelate-5PW columns utilize the tendency of iminodiacetic acid (IDA) to form a complex with metal ions, such as Zn2+, Ni2+ and Cu2+. The column is loaded with divalent metal ions by chelation. Peptides and proteins containing histidine residues will normally adsorb to these chelated ions at neutral pH. The retained compounds can be eluted with buffer containing imidazole or glycine. The key to making successful use of this retention mechanism is the proper selection of metal ions for chelation and the elution buffer to desorb the analytes. In general, Cu2+, interacts better with proteins; however, resolution is usually enhanced with Zn2+. A gradient mobile phase containing increasing imidazole or glycine concentrations is used to elute the retained compounds. Alternatively, a decreasing pH gradient can be used for elution purposes as well.
Applications include: immunoglobulins, transferrin, lectins, milk proteins, membrane proteins and peptides.
TSKgel Chelate-5PW columns utilize the tendency of iminodiacetic acid (IDA) to form a complex with metal ions, such as Zn2+, Ni2+ and Cu2+. The column is loaded with divalent metal ions by chelation. Peptides and proteins containing histidine residues will normally adsorb to these chelated ions at neutral pH. The retained compounds can be eluted with buffer containing imidazole or glycine. The key to making successful use of this retention mechanism is the proper selection of metal ions for chelation and the elution buffer to desorb the analytes. In general, Cu2+, interacts better with proteins; however, resolution is usually enhanced with Zn2+. A gradient mobile phase containing increasing imidazole or glycine concentrations is used to elute the retained compounds. Alternatively, a decreasing pH gradient can be used for elution purposes as well.
Applications include: immunoglobulins, transferrin, lectins, milk proteins, membrane proteins and peptides.
성분
TSKgel Guardgel kits contain:
Dimensions for Guardgel kits for 21.5 mm ID columns:
- 5 mL Guardgel packing material
- Column holder, consisting of external reducing union, cap, frit, 2 ferrules
- Column, 25 mm x 9.5 mm OD x 6 mm ID, 2 μm frit at 10 mm depth, 1/16"
- 2 Replacement frits
- Capillary tubing, 5 cm x 1/16" OD x 0.01"
- Nuts and ferrules, 10-32 threads for 1/16" tubing, 2 each
- All hardware is 316 stainless steel
Dimensions for Guardgel kits for 21.5 mm ID columns:
- Column, 35 mm x 12.75 mm OD x 10 mm ID, 10 μm frit at 20mm depth, 1/16"
- Capillary tubing, 5 cm x 1/16" OD x 0.02" ID
법적 정보
TSKgel is a registered trademark of Tosoh Corporation
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