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Merck
모든 사진(5)

주요 문서

ZRB2513

Sigma-Aldrich

Anti-MYH6 Antibody, clone 1L5 ZooMAb® Rabbit Monoclonal

newenhanced validation
greener alternative

recombinant, expressed in HEK 293 cells

동의어(들):

Myosin heavy chain 6, Myosin heavy chain, cardiac muscle alpha isoform (MyHC-alpha), Myosin-6

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About This Item

UNSPSC 코드:
12352203

생물학적 소스

rabbit

Quality Level

재조합

expressed in HEK 293 cells

결합

unconjugated

항체 형태

purified antibody

항체 생산 유형

primary antibodies

클론

1L5, recombinant monoclonal

설명

recombinant, expressed in HEK 293 cells

제품 라인

ZooMAb® learn more

형태

lyophilized

분자량

calculated mol wt 223.74 kDa
observed mol wt ~224 kDa

정제법

using Protein A

종 반응성

human

포장

antibody small pack of 25

환경친화적 대안 제품 특성

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

향상된 검증

recombinant expression
Learn more about Antibody Enhanced Validation

sustainability

Greener Alternative Product

기술

affinity binding assay: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable

동형

IgG

에피토프 서열

N-terminal half

단백질 ID 수납 번호

UniProt 수납 번호

환경친화적 대안 카테고리

배송 상태

ambient

저장 온도

2-8°C

유전자 정보

human ... MYH6(4624)

일반 설명

We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.

특이성

Clone 1L5 is a ZooMAb® recombinant rabbit monoclonal antibody that specifically detects MYH6. It targets an epitope within 17 amino acids from the N-terminal half.

면역원

KLH-conjugated linear peptide corresponding to 17 amino acids from the N-terminal half of human MYH6.

애플리케이션

Quality Control Testing

Evaluated by Western Blotting in human heart tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected MYH6 in human heart tissue lysate.

Tested Applications

Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected MYH6 in human heart tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected MYH6 in human heart tissue sections and human skeletal muscle tissue sections.

Affinity Binding Assay: A representative lot of this antibody bound MYH6 peptide with at least one hundred thousand-fold (100,000X) higher than with non-specific control peptide.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

표적 설명

Myosin-6 (UniProt: P13533; also known as Myosin heavy chain 6, Myosin heavy chain, cardiac muscle alpha isoform (MyHC-alpha)) is encoded by the MYH6 (also known as MYHCA) gene (Gene ID:4624) in human. MYH6 (myosin, heavy chain 6) gene encodes the heavy chain subunit of the cardiac muscle myosin, which is a hexamer made up of two heavy chain subunits, two light chain subunits, and two regulatory subunits. It is involved in the contraction of muscles. MYH6 is predominantly expressed in the heart, particularly in the atrial muscle. It is one of the major isoforms of cardiac myosin heavy chain, with its expression being more significant in the atria compared to the ventricles. MYH6 is localized in the sarcomere, the fundamental unit of muscle contraction, within the cardiac muscle cells. Specifically, it is a component of the thick filaments in the A-band of the sarcomere, where it interacts with actin filaments to facilitate muscle contraction. Defects in this gene cause atrial septal defect 3 (ASD3) characterized by incomplete closure of the wall between the atria resulting in blood flow from the left to the right atria. Its expression is also altered during thyroid hormone depletion and hemodynamic overloading. Mutations in MYH6 can disrupt the normal function of the protein, impairing cardiac muscle contraction and leading to several cardiac diseases. Mutations in this gene are associated with familial hypertrophic cardiomyopathy, dilated cardiomyopathy, and heart rhythm disorders due to malfunction of the sinus node, leading to irregular heartbeats. Genetic screening and molecular studies are crucial for identifying these mutations and understanding their impact on cardiac function. This ZooMAb® recombinant monoclonal antibody, generated by our proprietary technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

물리적 형태

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

재구성

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

저장 및 안정성

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

법적 정보

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

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