추천 제품
생물학적 소스
rabbit
Quality Level
재조합
expressed in HEK 293 cells
결합
unconjugated
항체 형태
purified antibody
항체 생산 유형
primary antibodies
클론
1E17, recombinant monoclonal
설명
1E17 Clone
제품 라인
ZooMAb® learn more
형태
lyophilized
분자량
calculated mol wt 114.90 kDa
observed mol wt ~160 kDa
정제법
using Protein A
종 반응성
human
종 반응성(상동성에 의해 예측)
monkey
포장
antibody small pack of 25 μL
환경친화적 대안 제품 특성
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
향상된 검증
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
기술
affinity binding assay: suitable
immunocytochemistry: suitable
peptide synthesis: suitable
western blot: suitable
동형
IgG
에피토프 서열
Internal
단백질 ID 수납 번호
UniProt 수납 번호
환경친화적 대안 카테고리
배송 상태
ambient
저장 온도
2-8°C
타겟 번역 후 변형
phosphorylation (pSer555)
유전자 정보
human ... ULK1(8408)
일반 설명
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
특이성
Clone 1E17 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects ULK1 phosphorylated on serine 555.
면역원
KLH-conjugated linear peptide corresponding to 10 amino acids surrounding phosphoserine 555 of human ULK1.
애플리케이션
Quality Control Testing
Evaluated by Western Blotting in MCF7 cells, overnight starved and treated with oligomycin.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected phospho-ULK1-Ser555 in MCF7 cells serum starved overnight and treated with Oligomycin (0.5 mM)
Tested applications
Peptide Inhibition Assay: Target band detection in lysates from MCF7 cells, serum starved and treated with oligomycin (0.5 mM), was prevented by preblocking of a representative lot with the immunogen phosphopeptide, but not the corresponding non-phosphopeptide.
Immunocytochemistry Analysis (ICC): A 1:100 dilution of this antibody detected phospho-ULK1-Ser555 in serum starved MCF7 cells.
Affinity Binding Assay: A representative lot of this antibody bound phospho-ULK1-Ser555 with a KD of 1.1 x 10-7 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in MCF7 cells, overnight starved and treated with oligomycin.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected phospho-ULK1-Ser555 in MCF7 cells serum starved overnight and treated with Oligomycin (0.5 mM)
Tested applications
Peptide Inhibition Assay: Target band detection in lysates from MCF7 cells, serum starved and treated with oligomycin (0.5 mM), was prevented by preblocking of a representative lot with the immunogen phosphopeptide, but not the corresponding non-phosphopeptide.
Immunocytochemistry Analysis (ICC): A 1:100 dilution of this antibody detected phospho-ULK1-Ser555 in serum starved MCF7 cells.
Affinity Binding Assay: A representative lot of this antibody bound phospho-ULK1-Ser555 with a KD of 1.1 x 10-7 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
표적 설명
Serine/threonine-protein kinase ULK1 (UniProt: O75385; also known as EC:2.7.11.1, Autophagy-related protein 1 homolog, ATG1, hATG1, Unc-51-like kinase 1) is encoded by the ULK1 (also known as KIAA0722) gene (Gene ID: 8408) in human. ULK1 is a ubiquitously expressed serine-threonine protein kinase that is involved in autophagy in response to starvation. During starvation it is localized to puncate structures primarily representing the isolation membrane that sequesters a portion of the cytoplasm resulting in the formation of an autophagosome. In cells, ULK1 is constitutively expressed in complex with at least three proteins: ATG13, FIP200 and ATG101. Interaction of ULK1 with ATG13 or FIP200 is reported to increase ULK1 kinase activity and stability. ULK1 undergoes autophosphorylation under nutrient-rich conditions and is dephosphorylated during starvation or following treatment with rapamycin. Under nutrient sufficiency, it is phosphorylated by MTOR/mTOR, which disrupts the interaction with AMPK and prevents activation of ULK1. In response to nutrient limitation, it is phosphorylated and activated by AMPK that leads to activation of autophagy. Phosphorylation at Ser555 by AMPK is reported to be critical for starvation-induced autophagy and for cell survival under conditions of low nutrients and energy. ULK1 can also act as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negative regulation of AMPK activity. Under nutrient sufficiency, high mTOR activity is reported to prevent ULK1 activation by phosphorylating ULK1 and disrupt its interaction with AMPK. The protein kinase domain of ULK1 is localized to amino acids 16-278. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Zachari, M., and Ganley, IG. (2017). Essays Biochem. 61(6); 585-596; Kim, J., et al. (2011). Nat. Cell Biol. 13(2); 132-141; Egan, DF., et al. (2011). Science. 331(6016). 456-461).
물리적 형태
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
재구성
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
저장 및 안정성
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
법적 정보
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
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