생물학적 소스
rabbit
Quality Level
재조합
expressed in HEK 293 cells
결합
unconjugated
항체 형태
purified antibody
항체 생산 유형
primary antibodies
클론
1D8, recombinant monoclonal
설명
recombinant, expressed in HEK 293 cells
제품 라인
ZooMAb® learn more
양식
lyophilized
분자량
calculated mol wt 21.87 kDa
observed mol wt ~31 kDa
정제법
using Protein A
포장
antibody small pack of 25 μL
환경친화적 대안 제품 특성
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
향상된 검증
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
기술
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
동형
IgG
단백질 ID 수납 번호
UniProt 수납 번호
환경친화적 대안 카테고리
, Aligned
배송 상태
ambient
저장 온도
2-8°C
타겟 번역 후 변형
unmodified
유전자 정보
human ... HIST1H1E(3008)
일반 설명
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
특이성
Clone 1D8 a ZooMAb® Mouse recombinant monoclonal antibody that specifically detects Histone H1 with H1E frameshift mutation.
면역원
KLH-conjugated linear peptide corresponding to a modified 12 amino acids sequence from the human Histone H1E.
애플리케이션
Quality Control Testing
Evaluated by Western Blotting in Human oligodendroglioma cells expressing wild-type, heterozygous fs, or homozygous fs H1E.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected Histone H1E frameshift in Human oligodendroglioma cells expressing heterozygous fs (B39), or homozygous fs (A41) H1E, but not the wild-type H1E.
Tested applications
Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected Histone H1E frameshift in Oligodendroglioma cells transfected with Histone H1E frameshift transgene (Data courtesy of Dr. Alexey Soshnev and Dr C. David Allis, Rockefeller University).
Chromatin Immunoprecipitation Analysis (ChIP): A representative lot detected Histone H1E frameshift in ChIP analysis of Oligodendroglioma cells transfected with Histone H1E frameshift or wildtype H1E transgenes (Data courtesy of Dr. Alexey Soshnev and Dr. C. David Allis, Rockefeller University).
Immunohistochemistry Applications: A representative lot detected H1E frameshift, clone 1D8 ZooMAbZooMAb® Rabbit Monoclonal in Immunohistochemistry applications Wildtype and Histone H1E Frameshift transgenic mouse brain (Data courtesy of Dr. Alexey Soshnev and Dr. C. David Allis, Rockefeller University).
Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected Histone H1E frameshift in Human blood, mouse embryonic stem cells (ESCs), and human oligodendroglioma cells expressing H1E frameshift (Data courtesy of Dr. Alexey Soshnev and Dr. C. David Allis, Rockefeller University).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in Human oligodendroglioma cells expressing wild-type, heterozygous fs, or homozygous fs H1E.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected Histone H1E frameshift in Human oligodendroglioma cells expressing heterozygous fs (B39), or homozygous fs (A41) H1E, but not the wild-type H1E.
Tested applications
Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected Histone H1E frameshift in Oligodendroglioma cells transfected with Histone H1E frameshift transgene (Data courtesy of Dr. Alexey Soshnev and Dr C. David Allis, Rockefeller University).
Chromatin Immunoprecipitation Analysis (ChIP): A representative lot detected Histone H1E frameshift in ChIP analysis of Oligodendroglioma cells transfected with Histone H1E frameshift or wildtype H1E transgenes (Data courtesy of Dr. Alexey Soshnev and Dr. C. David Allis, Rockefeller University).
Immunohistochemistry Applications: A representative lot detected H1E frameshift, clone 1D8 ZooMAbZooMAb® Rabbit Monoclonal in Immunohistochemistry applications Wildtype and Histone H1E Frameshift transgenic mouse brain (Data courtesy of Dr. Alexey Soshnev and Dr. C. David Allis, Rockefeller University).
Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected Histone H1E frameshift in Human blood, mouse embryonic stem cells (ESCs), and human oligodendroglioma cells expressing H1E frameshift (Data courtesy of Dr. Alexey Soshnev and Dr. C. David Allis, Rockefeller University).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
표적 설명
Histone 1, H1e (UniProt: A3R0T8; also known as Histone H1.4, HIST1H1E) is encoded by the HIST1H1E (also known as hCG_16457421) gene (Gene ID: 3008) in human. Histones have been classified under five major classes. The core histones H2A, H2B, H3, and H4 constitute the protein components of nucleosome core particles and histone H1 is a linker histone that plays a role in chromatin folding. Depletion of histone H1 can cause dramatic changes in chromatin structure, global nucleosome spacing, and reduced chromatin compaction. Pathogenic mutations in HIST1H1E gene result in the formation of histone H1 that is less effective in neutralizing negatively charged linker DNA and display reduced DNA binding and protein-protein interactions due to truncation in key residues. These mutations are reported to result in a shift in reading frame in the C-terminal region and produce truncated proteins. Frameshift mutations in HIST1H1E have been linked to Rahman syndrome that is manifested in overgrowth with intellectual disabilities (OGID) in early infancy. Subjects with Rahman syndrome display a global tendency in reduction of methylation state. This ZooMAbZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals (Ref.: Andrea Ciolfi, et al. (2020). Clinical Epigenetics 12; Article 7; Tatton-Brown , K., et al. (2017). Am. J. Hum. Genet. 100(5); 725-736).
물리적 형태
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
재구성
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
저장 및 안정성
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
법적 정보
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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