ZRB1091

Anti-GAD65 Antibody, clone 3B23 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• 동의어(들): 65 kDa glutamic acid decarboxylase, EC:4.1.1.15, GAD-65, Glutamate decarboxylase 2, Glutamate decarboxylase 65 kDa isoform
• UNSPSC 코드: 12352203
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• Quality Level: 200
• 재조합: expressed in HEK 293 cells
• 결합: unconjugated
• 항체 형태: purified antibody
• 항체 생산 유형: primary antibodies
• 클론: 3B23, recombinant monoclonal; monoclonal
• 제품 라인: ZooMAb^® learn more
• 형태: lyophilized
• 분자량: calculated mol wt 65.41 kDa; observed mol wt ~58 kDa
• 종 반응성: rat, mouse, human
• 포장: antibody small pack of 25 μL
• 환경친화적 대안 제품 특성: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• 향상된 검증: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• 기술: affinity binding assay: suitable; immunocytochemistry: suitable; immunofluorescence: suitable; immunohistochemistry: suitable; western blot: suitable
• UniProt 수납 번호: Q05329
• 환경친화적 대안 카테고리: , Aligned
• 배송 상태: ambient
• 저장 온도: 2-8°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... GAD2(2572)

설명

일반 설명

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

특이성

Clone 2B23 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Glutamate decarboxylase 2 (GAD65). It targets an epitope within 17 amino acids from the C-terminal region.

면역원

KLH-conjugated linear peptide corresponding to 17 amino acids from the C-terminal region of human Glutamate decarboxylase 2 (GAD65).

애플리케이션

Anti-GAD65, clone 3B23 ZooMAb, Cat. No. ZRB1091, is a recombinant Rabbit monoclonal antibody that targets GAD65 and is tested for use in Affinity Binding Assay, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, and Western Blotting.

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected GAD65 human and mouse brain tissue lysates.

Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected GAD65 in rat cerebellum tissue sections.

Affinity Binding Assay:: A representative lot of this antibody bound human GAD65 peptide with a KD of 1 x 10-12 in an affinity binding assay.

Immunohistochemistry (Paraffin) Analysis: A 1:10-100 dilution from a representative lot detected GAD65 in human brain and rat brain tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representativelot detected GAD65 in Neuro-2a cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

표적 설명

Glutamate decarboxylase 2 (UniProt: Q05329; also known as EC:4.1.1.15, 65 kDa glutamic acid decarboxylase, GAD-65, Glutamate decarboxylase 65 kDa isoform) is encoded by the GAD2 (GAD65) gene (Gene ID: 2572) in human. GAD-65 a homodimeric peripheral membrane protein that is associated to cytoplasmic vesicles. It is produced primarily by pancreatic islet cells. It is expressed in human β cells; however, some expression has also been observed in and cells of islets. It is shown to be a major target autoantigen in type 1A diabetes. In neurons, it is present in cytosolic leaflet of Golgi membranes and presynaptic clusters. It is responsible for the conversion of glutamic acid to gamma-aminobutyric acid (GABA), the major inhibitory transmitter in higher brain regions. Two molecular forms of GAD have been described (65 kDa and 67 kDa) that display 64% amino acid homology and both forms are expressed in the CNS, pancreatic islet cells, testis, oviduct, and ovary. Between the two isoforms, GAD67 is responsible for over 90% of basal GABA synthesis and is produced at limiting levels in the brain. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

물리적 형태

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

재구성

30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

저장 및 안정성

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

법적 정보

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1