T7915

Thioredoxin Reductase from Escherichia coli

ammonium sulfate suspension, >25 units/mg protein (Bradford)

• 동의어(들): NADPH:oxidized thioredoxin oxidoreductase
• CAS Number: 9074-14-0
• 효소 위원회 번호: 1.8.1.9 (BRENDA, IUBMB)
• MDL number: MFCD00678149
• UNSPSC 코드: 12352204
• NACRES: NA.32

속성

• 생물학적 소스: Escherichia coli
• Quality Level: 200
• 양식: ammonium sulfate suspension
• 특이 활성도: >25 units/mg protein (Bradford)
• 분자량: 70 kDa
• 기술: activity assay: suitable
• UniProt 수납 번호: P0A9P4
• 저장 온도: 2-8°C
• 유전자 정보: Escherichia coli K12 ... trxB(949054)

설명

일반 설명

Research area: Cell Signaling
Thioredoxin reductases (TrxRs) belongs to family of selenium-containing pyridine nucleotide-disulphide oxidoreductases.

애플리케이션

Thioredoxin Reductase from Escherichia coli can be used in peroxidase-coupled thioredoxin system assay for assessing the peroxidase activitiy of Cys-based thiol peroxidases.Thioredoxin Reductase from Escherichia coli has been used:

• for determining the enzymatic activity of His6-Ahp1p.
• to assay C. elegans TRXR using the TXN-dependent activity assay.
• to investigate the biological reducing systems for organic hydroperoxide resistance R gene (OhrR).
• for enzymatic targeting of auranofin to test its antimicrobial properties.
• in thioredoxin reductase activity and peroxiredoxin assay.
• to study the synergy between broccoli sprout extract and selenium in the upregulation of thioredoxin reductase in human hepatocytes.

Thioredoxin reductase from Escherichia coli has been used in thioredoxin reductase activity and peroxiredoxin assay.
It has also been used to study the synergy between broccoli sprout extract and selenium in the upregulation of thioredoxin reductase in human hepatocytes.

생화학적/생리학적 작용

An FAD-containing enzyme involved in the transfer of hydrogen from E. coli thioredoxin to other proteins thus providing a powerful disulfide reductase system.

Thioredoxin reductase (TrxR) is an NADPH-dependent oxidoreductase containing one FAD per subunit that reduces the active site disulfide in oxidised thioredoxin (Trx). The molecular weight of the isozymes from mammalian sources vary between 55-67 kDa as compared with 35 kDa in prokaryotes, plants or yeast. The substrate specificity of the mammalian enzyme is much broader than the prokaryotic enzyme reducing both mammalian and E. coli thioredoxins as well as well as non-disulfide substrates such selenite, lipoic acids, lipid hydroperoxides and hydrogen peroxide.

Thioredoxin reductase is a FAD containing enzyme, which transfers the reducing equivalent from NADPH to the disulphide bond of the enzyme by using FAD moiety within the Cys-Ala-Thr-Cys sequence. It can also reduce Trx-S2 to Trx-(SH)2 by using NADPH.

단위 정의

One unit will cause an increase in absorbance of 1.0 at 412 nm (when measured in a coupled assay with E. coli thioredoxin and DTNB) per min per mL at pH 7.0 at 25 °C.

물리적 형태

Suspension in 3.6 M (NH₄)₂SO₄ containing 30 mM potassium phosphate buffer, pH 7.5, and 2 mM EDTA.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable