T7777

Tris-Glycine-SDS Buffer 10× Concentrate

• 동의어(들): TRIS glycine SDS buffer solution
• UNSPSC 코드: 41105319
• eCl@ss: 32129211
• NACRES: NA.25

속성

• 무균: 0.2 μm filtered
• Quality Level: 200
• 양식: liquid
• 응용 분야: diagnostic assay manufacturing
• 저장 온도: 2-8°C

설명

일반 설명

Tris-glycine-SDS (TGS) running buffer is the most commonly used buffer for sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of proteins. TGS is usually used for both anode and cathode buffer. Recommended running conditions is 150 volts for mini vertical gel electrophoresis units.

애플리케이션

Tris-glycine-SDS buffer 10× concentrate has been used as a running buffer in sodium dodecyl sulfate-polyacryamide gel electrophoresis (SDS-PAGE). It is also used to block membranes with 5% non-fat dry milk for western blotting. TGS is usually used for both the anode buffer and the cathode buffer. Recommended running conditions is 150 volts for mini vertical gel electrophoresis units.

재구성

Dilution of the 10× TGS buffer produces a 1× running buffer containing 25 mM Tris, 192 mM glycine and 0.1% SDS, pH approx. 8.6.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type ABEK (EN14387) respirator filter