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T3411

Sigma-Aldrich

Tetramethylammonium chloride solution

for molecular biology

동의어(들):

N,N,N-Trimethylmethanaminium chloride

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About This Item

CAS Number:
75-57-0
MDL number:
MFCD00011628
UNSPSC 코드:
12352107
PubChem Substance ID:
24900143
NACRES:
NA.31

Grade

for molecular biology

Quality Level

200

농도

5 M

외래 활성

DNase, RNase, none detected

SMILES string

[Cl-].C[N+](C)(C)C

InChI

1S/C4H12N.ClH/c1-5(2,3)4;/h1-4H3;1H/q+1;/p-1

InChI key

OKIZCWYLBDKLSU-UHFFFAOYSA-M

일반 설명

Tetramethylammonium binds AT-rich DNA polymers while concomitantly abolishing the preferential melting of AT versus GC base pairs. It is supplied as a 0.2 μm filtered solution in 18 megohm water.

애플리케이션

Tetramethylammonium chloride solution (TMAC) has been used:
  • in the preparation of hybridization cocktail for array hybridization and scanning
  • in next-generation sequencing (NGS), and genome-wide unbiased identification of double-stranded breaks enabled by sequencing (GUIDE-seq) library preparation
  • in the preparation of TMAC buffer and bead hybridization mixture for hybridization and detection

시험 성적서(COA)

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문서 라이브러리 방문

W B Melchior et al.
Proceedings of the National Academy of Sciences of the United States of America, 70(2), 298-302 (1973-02-01)
Several small alkylammonium ions can eliminate, or even reverse, the usual dependence of the DNA transition temperature on base composition. For example, in 3 M tetramethylammonium chloride, or 2.4 M tetraethylammonium chloride, DNAs of different base compositions all melt at
Nikolay L Malinin et al.
Nature protocols, 16(12), 5592-5615 (2021-11-14)
Genome-wide unbiased identification of double-stranded breaks enabled by sequencing (GUIDE-seq) is a sensitive, unbiased, genome-wide method for defining the activity of genome-editing nucleases in living cells. GUIDE-seq is based on the principle of efficient integration of an end-protected double-stranded oligodeoxynucleotide
Hybridization of genomic DNA to oligonucleotide probes in the presence of tetramethylammonium chloride.
A G DiLella et al.
Methods in enzymology, 152, 447-451 (1987-01-01)
Xiao-Yong Li et al.
Methods in molecular biology (Clifton, N.J.), 809, 3-26 (2011-11-25)
Immunoprecipitation of cross-linked chromatin in combination with microarrays (ChIP-chip) or ultra high-throughput sequencing (ChIP-seq) is widely used to map genome-wide in vivo transcription factor binding. Both methods employ initial steps of in vivo cross-linking, chromatin isolation, DNA fragmentation, and immunoprecipitation.
Markus Welcker et al.
Genes & development, 27(23), 2531-2536 (2013-12-04)
The Fbw7 tumor suppressor targets a broad network of proteins for ubiquitylation. Here we show critical functions for Fbw7 dimerization in regulating the specificity and robustness of degradation. Dimerization enables Fbw7 to target substrates through concerted binding to two suboptimal
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