T2569

Trizma^® hydrochloride solution

pH 7.8, BioPerformance Certified, 1 M, suitable for cell culture

• 동의어(들): Tris hydrochloride solution
• UNSPSC 코드: 12161700
• NACRES: NA.25

속성

• Grade: BioPerformance Certified; for molecular biology
• Quality Level: 200
• 양식: solution
• 농도: 1 M
• 기술: cell culture | mammalian: suitable
• 불순물: DNase, RNase, Protease, none detected; bioburden, tested; endotoxin, tested; ≤5 ppm Heavy metals (as Pb)
• pH: 7.8
• 유용한 pH 범위: 7.0-9.0
• 흡수: ≤0.05 at 290 at 40%

설명

일반 설명

A series of Pre-mixed solutions of TRIZMA Base and TRIZMA HCl to provide commonly used pH values for Tris buffers. No mixing or pH adjustment necessary. Guaranteed accuracy ± 0.1 pH units.

Tris(hydroxymethyl)aminomethane (Tris) is a routine buffer in biological sciences. The pH buffering range is between 7.0 -9.0. It displays negligible binding towards metal in solutions. Tris is used majorly in combination with ethylenediaminetetraacetic acid (EDTA), especially for electrophoretic studies. Its low ionic mobility finds usage in capillary electrochromatography as well.

애플리케이션

Trizma^® hydrochloride solution has been used:

• as a component of lysis buffer for lysing human embryonic kidney (HEK) 293 cells
• in the 10X digoxigenin (DIG) deoxynucleoside triphosphate (dNTP) nick translation labeling buffer and in tris EDTA and sodium chloride (TEN) buffer
• in the preparation of tris, acetate, ethylenediaminetetraacetic acid (EDTA) (1x TAE) buffer and as a component of 10X gel loading dye

법적 정보

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type ABEK (EN14387) respirator filter