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Merck
모든 사진(1)

주요 문서

T1568

Sigma-Aldrich

Thrombopoietin human

recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture, ≥98% (SDS-PAGE and HPLC)

동의어(들):

MGDF, Megakaryocyte colony-stimulating factor, TPO, c-MPL ligand

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About This Item

CAS Number:
MDL number:
UNSPSC 코드:
12352202
NACRES:
NA.32

생물학적 소스

human

Quality Level

재조합

expressed in E. coli

분석

≥98% (SDS-PAGE and HPLC)

양식

lyophilized powder

효능

≤1.0 ng/mL ED50

분자량

18.6 kDa

포장

pkg of 5 μg

저장 조건

avoid repeated freeze/thaw cycles

기술

cell culture | mammalian: suitable

불순물

endotoxin, tested

UniProt 수납 번호

저장 온도

−20°C

유전자 정보

human ... THPO(7066)

유사한 제품을 찾으십니까? 방문 제품 비교 안내

애플리케이션

TPO stimulates the proliferation and maturation of megakaryocytes and promotes increased circulating levels of platelets in vivo. TPO signals through the c-mpl receptor and acts as an important regulator of circulating platelets

생화학적/생리학적 작용

TPO is a ligand for the c-mpl proto-oncogene (Mpl-L), a megakaryocyte colony stimulating factor and primary regulatory factor for megakaryocytopoiesis and thrombopoiesis. TPO is a humoral glycoprotein that stimulates growth and maturation of megakaryocytes and megakaryocytic colonies from bone marrow cultures.
Thrombopoeitin is a primary regulatory factor for megakaryocytopoiesis and thrombopoiesis. The mature form of TPO is a highly conserved glycoprotein, showing homology among various mammals. It is produced by liver and kidney cells. TPO stimulates growth and maturation of megakaryocytes and megakaryocytic colonies from bone marrow cultures. TPO binds and activates an 68-78 kDa glycoprotein receptor belonging to the GH family of cytokine receptors, a family that includes receptors to growth hormone (GH), erythropoietin (EPO), and prolactin (PRL). Like GH and EPO, TPO may bind to its receptor at two distinct sites, initiating receptor dimerization and activation. Analysis of mRNA indicates also the existence of a novel truncated and potentially soluble form of TPO receptor. The viral oncogene v-mlp of the myeloproliferative leukemia virus (MPLV) contains the gene sequence for the entire cytoplasmic and transmembrane domains and a portion of the extracellular domain of c-mlp (TPO receptor).

물리적 형태

Lyophilized from a 0.1 % TFA solution containing 250 μg bovine serum albumin

분석 메모

The biological activity is measured in a cell proliferation assay using MO7e cells.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, type N95 (US)


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