SRP7603

DNA Binding Protein-7 (DBP-7), recombinant

recombinant, expressed in E. coli, ≥99% (SDS-PAGE)

• 동의어(들): DNA Binding Protein 7
• UNSPSC 코드: 12352202
• NACRES: NA.32

속성

• 재조합: expressed in E. coli
• 분석: ≥99% (SDS-PAGE)
• 형태: lyophilized
• 분자량: ~9.4 kDa
• 포장: pkg of 20 μg
• 불순물: endotoxin, tested
• NCBI 수납 번호: P39476
• 배송 상태: wet ice
• 저장 온도: −20°C
• 유전자 정보: human ... sso7d(1454006)

설명

일반 설명

DNA Binding Protein-7 (DBP-7) is a N-terminal His-tagged recombinant protein and a member of the Sso7d family of small, abundant, non-specific DNA-binding proteins from the hyperthermophilic Archea Sulfolobus. The 7-kDa protein from Sulfolobus spp. consists of a five stranded, incomplete β-barrel capped at the opening by a C-terminal α-helix; they bind to the minor groove of a DNA duplex via the triple-stranded β-sheet. The topology of the Sulfolobus 7-kDa proteins was found to be similar to that of chromatin organization modifier (chromo) domains and eukaryotic SH3 domains, which are involved in protein–protein interactions. In vitro studies have shown that DBP-7 promotes the annealing of complementary DNA strands, induces negative supercoiling and chaperones the disassembly and renaturation of protein aggregates in an ATP hydrolysis-dependent manner.

물리적 형태

Sterile filtered and Lyophilized without additives.

재구성

Centrifuge the vial prior to opening. Avoid freeze-thaw cycles.

Reconstitute in water to a concentration of 0.1-1.0 mg/mL. The solution can then be diluted into other aqueous buffers.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable