SRP6491

ICAM1/CD54 human

recombinant, expressed in HEK 293 cells, ≥98% (SDS-PAGE)

• 동의어(들): BB-2, BB2, ICAM-1, P3.58
• UNSPSC 코드: 12352202
• NACRES: NA.32

속성

• 생물학적 소스: human
• 재조합: expressed in HEK 293 cells
• 분석: ≥98% (SDS-PAGE)
• 형태: lyophilized
• 분자량: calculated mol wt 75.7 kDa (This protein is fused with Fc fragment of human IgG1 at the C-terminus.); observed mol wt 100-110 kDa by SDS-PAGE (reducing) (Gln 27 is the predicted N-terminal.)
• 포장: pkg of 20 and 100 μg
• 불순물: <1 EU/μg endotoxin (LAL test)
• UniProt 수납 번호: P05362
• 배송 상태: wet ice
• 저장 온도: −20°C
• 유전자 정보: human ... ICAM1(3383)

설명

일반 설명

Inter-Cellular Adhesion Molecule 1 (ICAM-1), also known as Cluster of Differentiation 54 (CD54), is a member of the immunoglobulin superfamily, and is a cell surface glycoprotein which is typically expressed in low concentrations on endothelial cells and cells of the immune system. The protein encoded by this gene is a type of intercellular adhesion molecule continuously present in low concentrations in the membranes of leukocytes and endothelial cells. Upon cytokine stimulation, the concentrations greatly increase. ICAM-1 can be induced by interleukin-1 (IL-1) and tumor necrosis factor α (TNFα) and is expressed by the vascular endothelium, macrophages, and lymphocytes. ICAM-1 is a ligand for LFA-1 (integrin), a receptor found on leukocytes. When activated, leukocytes bind to endothelial cells via ICAM-1/LFA-1 and then transmigrate into tissues. ICAM-1 has been implicated in subarachnoid hemorrhage (SAH). Levels of ICAM-1 are shown to be significantly elevated in patients with SAH over control subjects in many studies. ICAM-1 expressed by respiratory epithelial cells is also the binding site for rhinovirus, the causative agent of most common colds.

물리적 형태

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM glycine, pH 7.0. Normally Mannitol or Trehalose is added as protectants before lyophilization.

재구성

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable