SRP5348

GST protein, tag-free

recombinant, expressed in E. coli, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

• CAS Number: 50812-37-8
• UNSPSC 코드: 12352202
• NACRES: NA.32

속성

• 재조합: expressed in E. coli
• 분석: ≥70% (SDS-PAGE)
• 양식: buffered aqueous glycerol solution
• 분자량: ~25 kDa
• 기술: ligand binding assay: suitable
• NCBI 수납 번호: U13850
• 배송 상태: dry ice
• 저장 온도: −70°C
• 유전자 정보: Escherichia coli IAI39 ... gst> gst; gst(7152360)

설명

일반 설명

Glutathione S-transferase (GST) family of enzymes comprises a long list of cytosolic, mitochondrial and microsomal proteins that are used to create the so-called GST gene fusion system. These proteins are capable of multiple reactions with a multitude of substrates. They have a role in detoxication and toxification mechanisms. GST tag has the size of 220 amino acids, which is relatively large compared to other tags like the myc- or the FLAG-tag that are quite small. GST can be used to purify and detect proteins of interest. In a GST gene fusion system, an expression vector consists of the gene sequence encoding the protein of interest and GST sequence alongside. After induction, the fusion proteins can be purified using affinity chromatography via its high affinity for glutathione.

애플리케이션

GST protein, tag-free has been used in in vitro F-actin binding assay. It has also been used as a protein tag for TPX2 (targeting protein for Xklp2).

물리적 형태

Supplied in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.

제조 메모

After opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable