SRP5011

CDK5/p35., active, GST tagged human

PRECISIO^® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

• 동의어(들): CDKN5, PSSALRE
• UNSPSC 코드: 12352200
• NACRES: NA.32

속성

• 재조합: expressed in baculovirus infected Sf9 cells
• 제품 라인: PRECISIO^® Kinase
• 분석: ≥70% (SDS-PAGE)
• 형태: buffered aqueous glycerol solution
• 특이 활성도: 157-213 nmol/min·mg
• 분자량: ~59 kDa (CDK5); ~60 kDa (p35)
• 기술: activity assay: suitable
• solubility: soluble; water: soluble
• NCBI 수납 번호: NM_003885; NM_004935
• 배송 상태: dry ice
• 저장 온도: −70°C
• 유전자 정보: human ... CDK5(1020) , CDK5R1(8851)

설명

일반 설명

Research area: Apoptosis

Cdk5 is a proline-directed serine/threonine protein kinase that belongs to the family of cyclin-dependent kinases (CDKs). Active CDK5, also known as neuronal cdc2-like kinase is a heterodimer of CDK5 and is essential for its kinase activity. They are found abundantly in the mammalian brain.

애플리케이션

CDK5 has been used as a component of a buffer during the measurement of the in vitro phosphorylation of peroxisome proliferator-activated receptor gamma (PPARγ).

생화학적/생리학적 작용

CDK5 is a neuron-specific kinase that plays a vital role in the development, functioning, and diseases of the central nervous system. Its optimum functioning is highly essential for proper neuronal migration, differentiation, axonal elongation, and synaptic function. CDK5 modulates neurite outgrowth, and axonal growth by interacting with several substrates like N- cadherin, Rap Guanine Nucleotide Exchange Factor 2(RapGEF2), focal adhesion kinase (FAK), p21 activating kinase 1, serotonin 6 receptor and Ras-related protein 8. CDK5 also plays a key role in the formation of the synapse, its maintenance, and communication. It is involved in the formation and retraction of the dendritic spines. It also regulates neuron secretion at the synapse by phosphorylating key mediators such as Synaspsin1. In addition, CDK5, present in the synaptic membrane phosphorylates the erythroblastic leukemia viral oncogene homolog B receptor (ErbB), resulting in increased acetylcholine receptor transcription. Lastly, CDK5 is also involved in the regulation of various other cellular processes like cell cycle, cell survival, apoptosis, gene regulation, etc.

물리적 형태

Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.

제조 메모

after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles

법적 정보

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable