SRP3283

FETUIN A human

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE), ≥95% (HPLC)

• 동의어(들): Alpha-2-HS-glycoprotein
• CAS Number: 9014-81-7
• UNSPSC 코드: 12352202
• NACRES: NA.32

속성

• 생물학적 소스: human
• 재조합: expressed in HEK 293 cells
• 분석: ≥95% (HPLC); ≥95% (SDS-PAGE)
• 양식: lyophilized
• 효능: ≥100 nM
• 분자량: 45.0-55.0 kDa
• 포장: pkg of 50 μg
• 불순물: <0.1 EU/μg endotoxin, tested
• 색상: white to off-white
• UniProt 수납 번호: P02765
• 배송 상태: wet ice
• 저장 온도: −20°C
• 유전자 정보: human ... AHSG(197)

설명

일반 설명

The gene AHSG (α-2-HS-glycoprotein, also referred to as fetuin-A) is mapped to human chromosome 3q27. Fetuin A/AHSG is a human plasma glycoprotein belonging to the Cystatin family of proteases inhibitors. Fetuin A is highly expressed in embryonic cells and adult hepatocytes, and is expressed to a lesser extent in monocytes/macrophages. The protein has cystatin-like domains, a calcium phosphate-binding site and a TGF (transforming growth factor)-β cytokine-binding motif. Recombinant human fetuin-A is a heterodimeric protein containing a 282 amino acid A chain and a 27 amino acid B chain. Due to glycosylation, recombinant fetuin-A migrates at an apparent molecular weight of 45–55kDa by SDS-PAGE run under nonreducing conditions.

애플리케이션

FETUIN A human has been used to stimulate mRNA expression in human monocytic THP1 cells, human in vitro differentiated adipocytes and C57BL/6 mice.

생화학적/생리학적 작용

AHSG (α-2-HS-glycoprotein, also referred to as fetuin-A) is a major serum protein component that exerts various calcium dependent physiological activities, and can mediate growth signaling in certain tumor cells. It also can act as a natural antagonist against specific TGF-β (transforming growth factor β) and BMP (bone morphogenetic protein) signaling proteins. High levels of fetuin-A in the serum is associated with mild osteogenesis imperfecta. On the other hand, reduced levels are seen in Paget′s disease. It is predicted as a potential biomarker for the early diagnosis of hypopharyngeal squamous cell carcinoma.

물리적 형태

Lyophilized from 10 mM Sodium Phosphate, pH 7.8.

재구성

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable