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Merck
모든 사진(1)

주요 문서

SRP3216

Sigma-Aldrich

KC (CXCL1) from mouse

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

동의어(들):

CXCL1, GRO1, GROa, NAP-3

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About This Item

UNSPSC 코드:
12352200
NACRES:
NA.32

생물학적 소스

mouse

재조합

expressed in E. coli

분석

≥98% (HPLC)
≥98% (SDS-PAGE)

양식

lyophilized

분자량

7.8 kDa

포장

pkg of 20 μg

기술

cell culture | mammalian: suitable

불순물

<0.1 EU/μg endotoxin, tested

색상

off-white to yellow

UniProt 수납 번호

배송 상태

wet ice

저장 온도

−20°C

유전자 정보

mouse ... CXCL1(14825)

일반 설명

C-X-C motif chemokine receptor 1 (CXCL1) is also known as keratinocyte-derived chemokine (KC). It is part of the glutamate-leucine-arginine (ELR)-containing CXC chemokine family. Recombinant Murine KC (CXCL1) is a 7.8kDa protein consisting of 72 amino acids including the ‘ELR′ motif common to the CXC chemokine family that bind to CXCR1 or CXCR2.

애플리케이션

C-X-C motif chemokine receptor 1 (CXCL1) from mouse has been used in transwell assay for chemotaxis.

생화학적/생리학적 작용

All three isoforms of growth-related protein (GRO) are CXC chemokines that can signal through C-X-C motif chemokine receptors 1 or 2 (CXCR1 or 2). The GRO proteins chemoattract and activate neutrophils and basophils. It has a role in host defense against polymicrobial sepsis, upregulation of intercellular adhesion molecule 1 (ICAM-1) and migration of neutrophils. The protein also activates mitogen-activated protein kinases (MAPKs), pro-inflammatory proteins and nuclear factor-κB (NF-κB).

서열

APIANELRCQ CLQTMAGIHL KNIQSLKVLP SGPHCTQTEV IATLKNGREA CLDPEAPLVQ KIVQKMLKGV PK

물리적 형태

Lyophilized with no additives.

재구성

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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시험 성적서(COA)

Lot/Batch Number

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문서 라이브러리 방문

Muscle-derived expression of the chemokine CXCL1 attenuates diet-induced obesity and improves fatty acid oxidation in the muscle.
Pedersen L
American Journal of Physiology. Endocrinology and Metabolism, 302(7), E831-E840 (2012)
CXCL1 contributes to host defense in polymicrobial sepsis via modulating T cell and neutrophil functions.
Jin L
Journal of Immunology, 193(7), 3549-3558 (2014)
GRO: a novel chemotactic cytokine.
R Sager et al.
Advances in experimental medicine and biology, 305, 73-77 (1991-01-01)
P Tekamp-Olson et al.
The Journal of experimental medicine, 172(3), 911-919 (1990-09-01)
A cDNA clone of murine macrophage inflammatory protein 2 (MIP-2) has been isolated from a library prepared from lipopolysaccharide (LPS)-stimulated RAW 264.7 cells and the nucleotide sequence determined. This cDNA was used to clone cDNAs for human homologues of MIP-2
S Haskill et al.
Proceedings of the National Academy of Sciences of the United States of America, 87(19), 7732-7736 (1990-10-01)
The product of the human GRO gene is a cytokine with inflammatory and growth-regulatory properties; GRO is also called MGSA for melanoma growth-stimulatory activity. We have identified two additional genes, GRO beta and GRO gamma, that share 90% and 86%

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