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Merck
모든 사진(1)

Key Documents

SRP3112

Sigma-Aldrich

MEC (CCL28) human

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

동의어(들):

CCK-1, CCL28, Mucosae-associated Epithelial Chemokine

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About This Item

UNSPSC 코드:
12352202
NACRES:
NA.32

생물학적 소스

human

재조합

expressed in E. coli

분석

≥98% (HPLC)
≥98% (SDS-PAGE)

형태

lyophilized

분자량

12.3 kDa

포장

pkg of 20 μg

기술

cell culture | mammalian: suitable

불순물

<0.1 EU/μg endotoxin, tested

색상

white to off-white

UniProt 수납 번호

배송 상태

wet ice

저장 온도

−20°C

유전자 정보

human ... CCL28(56477)

일반 설명

MEC is a secreted CC chemokine expressed primarily by epithelial cells of the bronchioles, salivary gland, mammary gland and colon. MEC signals through the CCR10 receptor and chemoattracts resting CD4, CD8 T-cells and eosinophils. MEC contains six cysteines including the four highly conserved cysteine residues present in CC chemokines. Recombinant human MEC is a 12.3 kDa protein containing 108 amino acid residues.

생화학적/생리학적 작용

MEC is a secreted CC chemokine expressed primarily by epithelial cells of the bronchioles, salivary gland, mammary gland and colon. Recombinant human MEC is a 12.3 kDa protein containing 108 amino acid residues.

서열

SEAILPIASS CCTEVSHHIS RRLLERVNMC RIQRADGDCD LAAVILHVKR RRICVSPHNH TVKQWMKVQA AKKNGKGNVC HRKKHHGKRN SNRAHQGKHE TYGHKTPY

물리적 형태

Lyophilized with no additives.

재구성

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

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문서 라이브러리 방문

J Pan et al.
Journal of immunology (Baltimore, Md. : 1950), 165(6), 2943-2949 (2000-09-07)
Mucosae-associated epithelial chemokine (MEC) is a novel chemokine whose mRNA is most abundant in salivary gland, with strong expression in other mucosal sites, including colon, trachea, and mammary gland. MEC is constitutively expressed by epithelial cells; MEC mRNA is detected
Eric J Kunkel et al.
Immunity, 16(1), 1-4 (2002-02-05)
Tissue-selective trafficking of memory and effector T and B lymphocytes is mediated by unique combinations of adhesion molecules and chemokines. The discovery of several related epithelial-expressed chemokines (TECK/CCL25 in small intestine, CTACK/CCL27 in skin, and MEC/CCL28 in diverse mucosal sites)
Emese Zsiros et al.
Clinical cancer research : an official journal of the American Association for Cancer Research, 21(12), 2840-2850 (2015-02-26)
Chemokines are implicated in T-cell trafficking. We mapped the chemokine landscape in advanced stage ovarian cancer and characterized the expression of cognate receptors in autologous dendritic cell (DC)-vaccine primed T cells in the context of cell-based immunotherapy. The expression of

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