SRP2093

SV40 T-antigen from simian virus

recombinant, expressed in insect cells, ≥80% (SDS-PAGE)

• UNSPSC 코드: 12352202
• NACRES: NA.77

속성

• 생물학적 소스: simian virus
• 재조합: expressed in insect cells
• 분석: ≥80% (SDS-PAGE)
• 형태: frozen liquid
• 분자량: ~83.3 kDa
• 포장: pkg of 5 μg
• 저장 조건: avoid repeated freeze/thaw cycles
• 농도: 400 μg/mL
• 색상: clear colorless
• NCBI 수납 번호: AAG39214.1
• UniProt 수납 번호: P03070
• 배송 상태: dry ice
• 저장 온도: −70°C
• 유전자 정보: simian virus ... SV40gp6(1489531)

설명

생화학적/생리학적 작용

The large T antigen (TAg) of Simian virus 40 acts as an initiator of DNA replication, participates in cellular transformation, and induces cell growth. A number of functional domains have been characterized including the N-terminal J domain, nuclear localization signal (NLS), internal helicase domain, Rb and topoisomerase I interacting domain, p53 and ATP binding domain. The p53-interacting domain is also essential for binding p300/CBP. Phosphorylation of TAg by several kinases may regulate its function both positively and negatively.

The viral replication of simian virus 40 (SV40) is carried out by a single protein, T-antigen. This feature makes the virus a useful system for understanding the process of replication and to identify the components of replication machinery. It functions as a DNA helicase along with a cellular single-strand DNA binding protein and topoisomerase I or II, leading to extensive origin unwinding and formation of a pre-initiation complex. The T-antigen associates with two tumor suppressor proteins, p53 and the retinoblastoma protein pRb, and inactivates them.

물리적 형태

Clear and colorless frozen liquid solution

제조 메모

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable