재조합
expressed in baculovirus infected Sf9 cells
분석
70-95% (SDS-PAGE)
양식
frozen liquid
특이 활성도
2.0-3.5 nmol/min·mg (radiometric)
분자량
~64 kDa (Cyclin Y)
~72 kDa (PFTK1 (CDK14))
농도
0.1 μg/μL
수납 번호(accession number)
NM_012395
NM_145012
배송 상태
dry ice
저장 온도
−70°C
유전자 정보
human ... CCNY(219771) , CDK14(5218)
애플리케이션
Useful for Kinase assay and Western blot.
생화학적/생리학적 작용
Recombinant human full-length PFTK1 (CDK14) and Cyclin Y (2-end) were co-expressed by Baculovirus in Sf9 insect cells using N-terminal GST tags. PFTK1 is a member of the CDC2 - related protein kinase family which is expressed primarily in the postnatal and adult nervous system (1). PFTK1 is expressed at high levels in brain, pancreas, kidney, heart, testis, and ovary. PFTK1 amino acid residue ser119 is required for its interaction with all four 14-3-3 isoforms, while ser120 may contribute slightly to PFTK1 binding (2). PFTK1 acts as a cyclin-dependent kinase that regulates cell cycle progression and cell proliferation. Overexpression of PFTK1 predicts resistance to chemotherapy in patients with oesophageal squamous cell carcinoma.
재구성
Formulated in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.
저장 및 안정성
Store in aliquots at -70°C. Avoid freeze/thaw cycles.
법적 정보
PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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