SRP0257

PDE1A Active human

recombinant, expressed in baculovirus infected insect cells, ≥70% (SDS-PAGE)

• 동의어(들): Calcium/calmodulin-dependent 3′,5′-cyclic nucleotide phosphodiesterase 1A, HDAM1, HSPDE1A, Phosphodiesterase 1A
• UNSPSC 코드: 12352200
• NACRES: NA.32

속성

• 생물학적 소스: human
• 재조합: expressed in baculovirus infected insect cells
• 분석: ≥70% (SDS-PAGE)
• 형태: aqueous solution
• 특이 활성도: ≥1237 units/μg
• 분자량: 88.3 kDa
• 포장: pkg of 10 μg
• 농도: >0.02 mg/mL
• NCBI 수납 번호: NM_005019
• UniProt 수납 번호: P54750
• 배송 상태: dry ice
• 저장 온도: −70°C
• 유전자 정보: human ... PDE1A(5136)

설명

일반 설명

Human PDE1A (GenBank Accession No. NM_005019), full length with N-terminal GST tag, MW = 88.3 kDa, expressed in a Baculovirus infected Sf9 cell expression system

애플리케이션

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

단위 정의

One unit is defined as the amount of enzyme that will convert 1 pmole of 3′, 5′-cAMP to 5′ AMP per min at 37°C

물리적 형태

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 20% glycerol and 3 mM DTT.

제조 메모

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.