SRP0169

JMJD2B Active human

recombinant, expressed in baculovirus infected insect cells, ≥40% (SDS-PAGE)

• 동의어(들): JHDM3B, JmjC domain-containing histone demethylation protein 3B, Jumonji domain-containing protein 2B, KIAA0876, Lysine-specific demethylase 4B (KDM4B)
• UNSPSC 코드: 12352202
• NACRES: NA.32

속성

• 생물학적 소스: human
• 재조합: expressed in baculovirus infected insect cells
• 분석: ≥40% (SDS-PAGE)
• 형태: aqueous solution
• 분자량: 57 kDa
• 포장: pkg of 100 μg
• 농도: >0.02 mg/mL
• NCBI 수납 번호: NM_015015
• UniProt 수납 번호: O94953
• 배송 상태: dry ice
• 저장 온도: −70°C
• 유전자 정보: human ... KDM4B(23030)

설명

일반 설명

Human JMJD2B, also known as JHDM3B and KDM4B (GenBank Accession No.NM_015015) (amino acids 1-500) with C-terminal FLAG tag, MW = 57 kDa, expressed in Sf9 cells using a Baculovirus expression system.

애플리케이션

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

단위 정의

One unit is defined as the amount of enzyme required to demethylate 1 pmol of substrate/min at 37°C.

물리적 형태

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 20% glycerol and 3 mM DTT.

제조 메모

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.