SRP0131

EZH1/EED/SUZ12/RbAp48/AEBP2 human

recombinant, expressed in baculovirus infected insect cells, ≥90% (SDS-PAGE)

• 동의어(들): ENX-2, EZH1, Enhancer of zeste homolog 1, KMT6B, Lysine N-methyltransferase 6B
• UNSPSC 코드: 12352202
• NACRES: NA.32

속성

• 생물학적 소스: human
• 재조합: expressed in baculovirus infected insect cells
• 분석: ≥90% (SDS-PAGE)
• 형태: aqueous solution
• 분자량: 48 kDa (RbAp48); 51 kDa (EED); 53 kDa (AEBP2); 86 kDa (EZH1); 87 kDa (SUZ12)
• 포장: pkg of 50 μg
• 농도: >0.02 mg/mL
• NCBI 수납 번호: NM_001991 (EZH1); NM_003797 (EED); NM_005610 (RbAP48); NM_015355 (SUZ12); NM_153207 (AEBP2)
• UniProt 수납 번호: Q92800
• 배송 상태: dry ice
• 저장 온도: −70°C
• 유전자 정보: human ... EZH1(2145)

설명

일반 설명

Complex of: Human EZH1, GenBank Accession No. NM_001991, amino acids 2-end, with N-terminal His tag, MW = 86 kDa. Human EED, NM_003797, amino acids 2-end, with N-terminal DDDDK tag (FLAG), MW = 51 kDa. Human SUZ12, NM_015355, amino acids 2-end, with N-terminal His tag. RbAp48 and AEBP2.

애플리케이션

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

단위 정의

One unit is defined as the amount of enzyme required to methylate 1 pmol of substrate/min at 37°C.

물리적 형태

Formulated in 25 mM Tris-HCl, pH 8.0, 138 mM NaCl, 0.05% Tween-20 and 10% glycerol.

제조 메모

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.