SRP0113

HDAC-11 human

recombinant, expressed in baculovirus infected insect cells, ≥10% (SDS-PAGE)

• 동의어(들): HD11, Histone deacetylase 11
• UNSPSC 코드: 12352200
• NACRES: NA.77

속성

• 생물학적 소스: human
• 재조합: expressed in baculovirus infected insect cells
• 분석: ≥10% (SDS-PAGE)
• 형태: aqueous solution
• 분자량: 66 kDa
• 포장: pkg of 50 μg
• 저장 조건: avoid repeated freeze/thaw cycles
• 농도: >0.02 mg/mL
• NCBI 수납 번호: BC009676
• UniProt 수납 번호: Q96DB2
• 배송 상태: dry ice
• 저장 온도: −70°C
• 유전자 정보: human ... HDAC11(79885)

설명

일반 설명

HDAC-11 (histone deacetylase 11) is a zinc-dependent HDAC. It belongs to the HDAC family of proteins and HDAC class IV. It is highly conserved among various species. The mRNA is present in the brain, skeletal muscle, kidney and testis. The gene is mapped to human chromosome 3p25.
Human HDAC11, full length, GenBank Accession No. BC009676, with N-terminal GST tag, MW = 66kDa, expressed in baculovirus expression system.

애플리케이션

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

생화학적/생리학적 작용

HDAC-11 (histone deacetylase 11) is responsible for deacetylation of nuclear histone and nonhistone proteins, including transcription factors. HDAC-11 catalyzes the deacetylation of histone H4. It binds to CDT1 (DNA replication factor) and is present with S phase-associated replication origins. HDAC-11 is upregulated in various carcinomas. Absence of it results in cell death and suppresses metabolic activity in colon, prostate, breast and ovarian cancer cell lines.

단위 정의

One unit is defined as the amount of enzyme required to deacetylate 1 pmol of substrate/min at 37°C.

물리적 형태

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20 and 50% glycerol.

제조 메모

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable