SRE0117

Salt Active Nuclease (SAN)

High Purity, recombinant, expressed in Pichia pastoris, Triton-free

• 동의어(들): endonuclease, salt active endonuclease, salt tolerant nuclease
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 재조합: expressed in Pichia pastoris
• Quality Level: 200
• 구성: Triton-free
• 포장: pkg of 25 kU
• 배송 상태: dry ice
• 저장 온도: −20°C

설명

일반 설명

This Salt Active Nuclease (SAN) is the ultimate solution for efficient removal of nucleic acids in manufacturing and bioprocessing workflows. This nonspecific, recombinant endonuclease has optimum activity at high salt concentrations, which can improve efficiency and yield in various workflows.

애플리케이션

• Useful for purification of tagged recombinant proteins
• Manufacturing of nucleic acid-free molecular enzymes and reagents
• Research of vaccines, viruses and antibodies

특징 및 장점

• Active at low temperatures
• Binds strongly to cation exchange media because of high isoelectric point, even at elevated pH and salt concentrations where few other proteins show binding
• Typically does not bind to anion exchange or IMAC media
• Detergent free No protease detected

단위 정의

One unit is defined as the amount of enzyme that causes a ΔA260 = 1.0 in 30 minutes at 37 °C in 25 mM Tris-HCI pH 8.5 (@25 °C), 5 mM MgCl₂, 500 mM NaCl, and 50 µg/mL calf thymus DNA.

재구성

In solution

기타 정보

• Temperature: 5 – 40°C, optimal: ~35°C
• Salt concentration (NaCl / KCl): 150 - 900 mM, optimal: 400 - 650 mM
• Mg²⁺: >1 mM is required for activity, optimal 5 - 50 mM
• pH: 7.3 – 10.0, optimal 8.2 - 9.2

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable