추천 제품
생물학적 소스
alpaca
Quality Level
재조합
expressed in E. coli
결합
unconjugated
항체 형태
affinity purified VHH antibody
클론
GE10, monoclonal
분석
>95% (SDS-PAGE)
양식
lyophilized solid
분자량
13.7 kDa
~15-18 kDa (SDS PAGE under reducing condition)
정제법
immobilized metal affinity chromatography (IMAC)
종 반응성
SARS coronavirus
농도
1 mg/mL
기술
ELISA: 1:2000-1:10000
immunofluorescence: 1:100-1:600
immunoprecipitation (IP): 0.5-4.0 μg
western blot: 1:1000-1:5000
색상
colorless
pH
( in PBS, pH 7.4)
동형
VHH
단백질 ID 수납 번호
UniProt 수납 번호
배송 상태
ambient
저장 온도
2-8°C
타겟 번역 후 변형
unmodified
일반 설명
Green fluorescent protein (GFP) is a protein composed of 238 amino acid residues(26.9kDa) derived from the Jellyfish Aequorea victoria, which emits green light (emission peak at 509nm) when excited by blue light (excitation peak at 395nm). GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. EGFP contains the double-amino-acid substitutions Phe-64 to Leu and Ser-65 to Thr (previously published as GFPmut1; PMID: 8707053). In contrast to wtGFP, EGFP has a single, strong, red-shifted excitation peak at 488 nm. Alpaca derived anti-GFP VHH single-domain antibody (molecular weight: 13.7 kDa), expressed in E. coli under conditions free from animal derived components.It has a 6His tag at its C-terminal.
Single-domain antibody (sdAb), also called nano-antibody, is the variable region isolated from the antigen-binding variable domain (VHH) of camelid heavy-chain-only antibody. It lacks light chains and a first constant domain(CH1). This configuration confers a number of unique advantages over conventional monoclonal antibodies. The small size of just 15 kDa (2~4 nm), single-domain antibody is the smallest antigen-binding fragment that can easily penetrate tissues, allowing them to bind epitopes that are inacessible to conventional antibodies. These single-domain antibodies offer superior chemical and thermal stability and increased solubility than conventional antibodies. All our antibodies are tested to ensure the highest level of specificity, sensitivity and reproducibility. Alpaca derived anti-GFP VHH single-domain antibody (molecular weight: 13.7 kDa), expressed in E. coli under conditions free from animal derived components.
Single-domain antibody (sdAb), also called nano-antibody, is the variable region isolated from the antigen-binding variable domain (VHH) of camelid heavy-chain-only antibody. It lacks light chains and a first constant domain(CH1). This configuration confers a number of unique advantages over conventional monoclonal antibodies. The small size of just 15 kDa (2~4 nm), single-domain antibody is the smallest antigen-binding fragment that can easily penetrate tissues, allowing them to bind epitopes that are inacessible to conventional antibodies. These single-domain antibodies offer superior chemical and thermal stability and increased solubility than conventional antibodies. All our antibodies are tested to ensure the highest level of specificity, sensitivity and reproducibility. Alpaca derived anti-GFP VHH single-domain antibody (molecular weight: 13.7 kDa), expressed in E. coli under conditions free from animal derived components.
특이성
Green fluorescence protein (GFP) and other derivatives of GFP
면역원
Recombinant full length GFP protein
애플리케이션
ELISA, WB, IP, IF
포장
50 µg per vial / 100 µg per vial
물리적 형태
Affinity purified VHH single domain antibody lyophilized from PBS, pH7.4
재구성
Reconstitute the lyophilized antibody with sterile deionized water or with 50% glycerol (ACS grade or better) to a stock solution of 1 mg/mL. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Avoid vigorous shaking or vortexing.
저장 및 안정성
Stable for 1 year at 4°C from date of receipt.
기타 정보
Storage at 4°C; Do not freeze.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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