SAB4702058

Monoclonal Anti-CD45 APC-Cyanine7 produced in mouse

• 동의어(들): B220, GP180, LCA, LY5, T200, TPC
• UNSPSC 코드: 12352203

속성

• 결합: APC-Cy7 conjugate
• Quality Level: 100
• 항체 생산 유형: primary antibodies
• 클론: MEM-28, monoclonal
• 양식: buffered aqueous solution
• 종 반응성: human
• 기술: flow cytometry: suitable
• 동형: IgG1
• UniProt 수납 번호: P08575
• 저장 온도: 2-8°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... PTPRC(5788)

설명

일반 설명

CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing; these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis.

특이성

The antibody MEM-28 reacts with an extracellular epitope on all alternative forms of human CD45 antigen (Leukocyte Common Antigen), a 180-220 kDa single chain type I transmembrane protein expressed at high level on all cells of hematopoietic origin, except erythrocytes and platelets.

면역원

Human thymocytes and T lymphocytes.

물리적 형태

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

제조 메모

Purified antibody is conjugated with activated tandem dye of allophycocyanin-cyanine 7 (APC-Cy7) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

저장 및 안정성

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

기타 정보

The antibody solution should be gently mixed before use.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable