SAB4200880

Anti-Cellular Fibronectin antibody, Mouse monoclonal

clone FN-3E2, purified from hybridoma cell culture

• 동의어(들): cFN
• UNSPSC 코드: 12352203
• NACRES: NA.41

속성

• 항체 형태: purified from hybridoma cell culture
• Quality Level: 200
• 클론: FN-3E2
• 양식: liquid
• 종 반응성: mouse, rat, human, monkey, chicken
• 농도: ~1 mg/mL
• 동형: IgM
• 배송 상태: dry ice
• 저장 온도: −20°C
• 타겟 번역 후 변형: unmodified

설명

일반 설명

Fibronectin is an extracellular matrix protein composed of two nearly identical disulfide-bound polypeptides with typical molecular weights of 220 - 240 kDa.1 Cellular fibronectin is structurally and antigenically similar to cold insoluble globulin from plasma.

특이성

Monoclonal Anti-Cellular Fibronectin labels fibronectin in the pericellular extracellular matrix of cultured chicken fibroblasts. It also labels cellular fibronectin in chicken embryo, mouse and human fibroblasts grown in tissue culture using in indirect immunofluorescent labeling technique.

애플리케이션

The antibody may also be used in immunoperoxidase or immunofluorescent labeling on frozen human tissue preparations. In immunoblotting, Monoclonal Anti-Cellular Fibronectin localizes the 240 kDa bands of cellular fibronectin in both its native and denatured forms.

생화학적/생리학적 작용

Analysis of the fibronectin molecule indicates that it contains several functionally and structurally distinct domains, which may bind to cell surfaces, collagen, heparin, etc.2,3,4,5. Numerous studies have shown the fibronectin may enhance cell adhesion and spreading and affect the routes of cell migration both in vivo and in culture.6 It has been shown that upon malignant transformation many cells lose their surface bound fibronectin.7

물리적 형태

Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.

저장 및 안정성

For continuous use, store at 2-8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours. Protect from prolonged exposure to light.

면책조항

This product is for R&D use only, not for drug, household, or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1