SAB4200842

Anti-PMEL antibody, Mouse monoclonal

clone PL-19, purified from hybridoma cell culture

• UNSPSC 코드: 12352203
• NACRES: NA.41

속성

• 항체 형태: purified from hybridoma cell culture
• Quality Level: 200
• 항체 생산 유형: primary antibodies
• 클론: PL-19
• 형태: liquid
• 종 반응성: human
• 농도: ~1 mg/mL
• 기술: immunoblotting: 1-2 μg/mL using human melanoma SK-MEL-28 cell lysate; immunofluorescence: 1-2 μg/mL using human melanoma SK-MEL-28 cells
• 동형: IgG1
• UniProt 수납 번호: P40967
• 배송 상태: dry ice
• 저장 온도: −20°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... PMEL(6490)

설명

일반 설명

The melanocyte-specific protein PMEL (also known as Pmel17, gp100, melanocyte protein, premelanosome protein or silver locus protein homolog (SILV) is a type I transmembrane glycoprotein that is expressed primarily in pigment cells of the eye and skin. In melanosomes, PMEL protein forms a fibrillar matrix on which the UV-shielding pigment melanin is deposited.1-2

특이성

Monoclonal Anti-PMEL antibody specifically recognizes human PMEL protein.

애플리케이션

The antibody may be used in various immunochemical techniques including Immunoblotting (~100 kDa), Immunohistochemistry and Immunofluorescence.

생화학적/생리학적 작용

PMEL fibrils are a major functional component of the melanosomal compartment as they optimize the melanin polymerization, condensation and storage.1,3
In addition, PMEL fibrils have an amyloidogenic nature sharing features with pathological amyloids.4 Thus, PMEL is suggested as an excellent model system to study mechanisms of intracellular amyloid formation.1
Mutations in PMEL are associated with pigmentation disorders and/or impairments in eye development in various species.1,5,6

물리적 형태

Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.

저장 및 안정성

For continuous use, store at 2-8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

면책조항

Unless otherwise stated in our catalog  our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable