추천 제품
제품명
Anti-Fibronectin antibody, Mouse monoclonal, clone FN-15, purified from hybridoma cell culture
생물학적 소스
mouse
Quality Level
항체 형태
purified from hybridoma cell culture
항체 생산 유형
primary antibodies
클론
FN-15, monoclonal
양식
buffered aqueous solution
분자량
220-260 kDa
종 반응성
human, mouse
농도
~1 mg/mL
기술
immunoblotting: 0.3-0.6 μg/mL using human fetal fibroblast-like WI-38 cell line extract
immunofluorescence: 2.5-5 μg/mL using human foreskin fibroblast Hs68 cells
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: 0.15-0.3 μg/mL using 5 μg/mL Fibronectin from human plasma for coating
동형
IgG1
UniProt 수납 번호
배송 상태
dry ice
저장 온도
−20°C
타겟 번역 후 변형
unmodified
유전자 정보
human ... FN1(2335)
일반 설명
Monoclonal Anti-Maltose Binding Protein (MBP) (mouse IgG1 isotype) is derived from the hybridoma MBP-17 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a purified recombinant MBP fusion protein. Fibronectin (FN), also known as Cold-insoluble globulin (CIG), is a multi-domain glycoprotein composed of two nearly identical disulfide-bound polypeptides. It is widely expressed by multiple cell types and is critically important in vertebrate development, as demonstrated by the early embryonic lethality of mice with targeted inactivation of the FN1 gene 6. In vertebrates, two types of Fibronectin are present: soluble plasma Fibronectin and insoluble cellular Fibronectin. The plasma form of Fibronectin is synthesized by hepatocytes, secreted to blood and, upon tissue injury, is incorporated into fibrin clots to exert effects on platelet function and to mediate hemostasis. Cellular Fibronectin is synthesized by many cell types, including fibroblasts, endothelial cells, chondrocytes, synovial cells and myocytes, it is assembled by cells as they migrate into the clot to reconstitute damaged tissue.
특이성
Anti-Fibronectin antibody, Mouse monoclonal specifically recognizes Fibronectin from plasma and in the pericellular extracellular matrix of cultured fibroblasts from human and mouse origin.
면역원
Fibronectin isolated from human plasma.
애플리케이션
The antibody may be used in various immunochemical techniques including Immunoblotting (∼220-260kDa)1, ELISA, Immunofluorescence, Immunohistochemistry and Immunoprecipitation.
생화학적/생리학적 작용
The plasma form of Fibronectin is synthesized by hepatocytes, secreted to blood and, upon tissue injury, is incorporated into fibrin clots to exert effects on platelet function and to mediate hemostasis. Cellular Fibronectin is synthesized by many cell types, including fibroblasts, endothelial cells, chondrocytes, synovial cells and myocytes, it is assembled by cells as they migrate into the clot to reconstitute damaged tissue.
물리적 형태
Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.
기타 정보
In order to obtain best results in different techniques and preparations we recommend determining optimal working concentration by titration test.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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이미 열람한 고객
E L George et al.
Development (Cambridge, England), 119(4), 1079-1091 (1993-12-01)
To examine the role of fibronectin in vivo, we have generated mice in which the fibronectin gene is inactivated. Heterozygotes have one half normal levels of plasma fibronectin, yet appear normal. When homozygous, the mutant allele causes early embryonic lethality
R Manabe et al.
The Journal of cell biology, 139(1), 295-307 (1997-10-06)
Fibronectin (FN) has a complex pattern of alternative splicing at the mRNA level. One of the alternatively spliced segments, EDA, is prominently expressed during biological processes involving substantial cell migration and proliferation, such as embryonic development, malignant transformation, and wound
Yong Mao et al.
Matrix biology : journal of the International Society for Matrix Biology, 24(6), 389-399 (2005-08-03)
The extracellular matrix provides a framework for cell adhesion, supports cell movement, and serves to compartmentalize tissues into functional units. Fibronectin is a core component of many extracellular matrices where it regulates a variety of cell activities through direct interactions
Mengmeng Fan et al.
Oncology reports, 47(1) (2021-11-06)
The epithelial‑stromal interaction 1 gene (EPSTI1) is known to play multiple roles in the malignant progression of breast cancer and also in some aspects of the immune responses to the tumor. However, the relevance of the gene in the onset/progression
Maria Zingariello et al.
American journal of blood research, 5(2), 34-61 (2015-01-01)
Despite numerous circumstantial evidences, the pathogenic role of TGF-β in primary myelofibrosis (PMF), the most severe of the Philadelphia-negative myeloproliferative neoplasms, is still unclear because of the modest (2-fold) increases in its plasma levels observed in PMF patients and in
Global Trade Item Number
| SKU | GTIN |
|---|---|
| SAB4200760-100UL | 4061833253779 |
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