SAB4200747

Anti-Neurofilament 200 antibody, Mouse monoclonal

clone NE14, purified from hybridoma cell culture

• 동의어(들): Anti-H-subunit, Anti-NF-H
• UNSPSC 코드: 12352203
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 200
• 항체 형태: purified from hybridoma cell culture
• 항체 생산 유형: primary antibodies
• 클론: NE14, monoclonal
• 형태: buffered aqueous solution
• 분자량: ~200 kDa
• 종 반응성: feline, rat, guinea pig, pig, human, mouse, bovine, chicken
• 농도: ~1.0 mg/mL
• 기술: immunoblotting: 1.25-2.5 μg/mL using rat brain S1 fraction; immunohistochemistry: 5-10 μg/mL using enzyme treated formalin-fixed, paraffin-embedded rat brain or mouse brain sections
• 동형: IgG1
• UniProt 수납 번호: P12036
• 배송 상태: dry ice
• 저장 온도: −20°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: bovine ... Nefh(528842) ; cat ... Nefh(101087272) ; chicken ... Nefh(417020) ; human ... NEFH(4744) ; mouse ... Nefh(380684) ; pig ... Nefh(100156492) ; rat ... Nefh(24587)

설명

일반 설명

Anti-Neurofilament 200 Antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the NE14 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Neurofilaments are built from three intertwined protofibrils of apparent molecular weights [68 (L), 160 (M) and 200 (H) kDa] which are themselves composed of two tetrameric protofilament complexes of monomeric proteins. Neurofilament 200 also known as neurofilament heavy polypeptide (Hsubunit), NF-H, NEFH or 200 kDa neurofilament protein.

면역원

Neurofilaments purified from pig spinal cord

애플리케이션

Anti-Neurofilament 200 antibody, Mouse monoclonal has been used in immunoblotting and immunohistochemistry staining.

생화학적/생리학적 작용

Neurofilaments are the type of intermediate filaments (IFs), that serve as major elements of the cytoskeleton supporting the axon cytoplasm of neuronal cells. It has an important function in mature axons that is not sub served by the two smaller neurofilament proteins. Defects in Neurofilament 200 are a cause of susceptibility to amyotrophic lateral sclerosis (ALS) and these accumulations are a hallmark of pathological lesion. Neurofilaments can accumulate in large numbers within cell bodies and proximal axons of affected neurons in several pathological diseases, such as Charcot-Marie-Tooth (CMT), neurofilament inclusion disease (NFID), giant axonal neuropathy (GAN), diabetic neuropathy, spinal muscular atrophy (SMA) and spastic paraplegia. In addition, neurofilament accumulations was detected in Alzheimer′s (AD) and Parkinson′s disease (PD) patients.

물리적 형태

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable