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Merck
모든 사진(2)

주요 문서

SAB4200746

Sigma-Aldrich

Anti-α-Fetoprotein (AFP) antibody, Mouse monoclonal

clone C3, purified from hybridoma cell culture

동의어(들):

Anti-AFP, Anti-Alpha-1-fetoprotein, Anti-Alpha-fetoglobulin

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About This Item

UNSPSC 코드:
12352203
NACRES:
NA.46

생물학적 소스

mouse

Quality Level

결합

unconjugated

항체 형태

purified from hybridoma cell culture

항체 생산 유형

primary antibodies

클론

C3, monoclonal

양식

buffered aqueous solution

분자량

~74 kDa

종 반응성

human, canine, porcine

농도

~1.0 mg/mL

기술

ELISA: suitable
dot blot: suitable
immunoblotting: 2-4 μg/mL using liver hepatocellular carcinoma HepG2 cell line extract
immunofluorescence: suitable
immunohistochemistry: 2-4 μg/mL using formalin-fixed, paraffin-embedded human fetal liver sections

동형

IgG2a

UniProt 수납 번호

배송 상태

dry ice

저장 온도

−20°C

타겟 번역 후 변형

unmodified

유전자 정보

human ... AFP(174)

일반 설명

The gene encoding α-fetoprotein (AFP) is localized on human chromosome 4. It encodes a plasma glycoprotein that has a molecular mass of 70kDa. It is synthesized along with serum albumin during the development of embryonic yolk sac and the liver.

면역원

Purified human α-fetoprotein

생화학적/생리학적 작용

α-fetoprotein (AFP) is involved in the regulation of fatty acids in both fetal and proliferating adult liver cells. Its expression is found to be increased in acute liver injuries, indicating active liver regeneration. AFP has been associated with fatty liver disease (FLD), a disease that may lead to cirrhosis and hepatocellular carcinoma. It serves as a tumor marker for HCC (hepatocellular carcinoma).

물리적 형태

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

저장 및 안정성

Store at –20°C. For continuous use, store at 2–8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

기타 정보

In order to obtain best results in different techniques and preparations, we recommend determining optimal working concentration by titration test.

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Storage Class Code

10 - Combustible liquids

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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문서 라이브러리 방문

Association between serum alpha-fetoprotein levels and fatty liver disease: a cross-sectional study.
Xu P, et al.
World Journal of Gastrointestinal Oncology (2014)
S Kate Alldred et al.
The Cochrane database of systematic reviews, (12), CD011984-CD011984 (2015-12-15)
Down's syndrome occurs when a person has three copies of chromosome 21, or the specific area of chromosome 21 implicated in causing Down's syndrome, rather than two. It is the commonest congenital cause of mental disability and also leads to
Evaluation of alpha-fetoprotein in detecting hepatocellular carcinoma recurrence after radiofrequency ablation.
Siripongsakun S, et al.
Journal of Gastroenterology and Hepatology (2014)
Clinical significance of elevated serum alpha-fetoprotein (AFP) level in acute viral hepatitis A (AHA).
Seo SI, et al.
Hepato-Gastroenterology (2013)
Bérénice Charrière et al.
World journal of hepatology, 8(21), 881-890 (2016-08-02)
Alpha-fetoprotein (AFP) is the main tumor biomarker available for the management of hepatocellular carcinoma (HCC). Although it is neither a good screening test nor an accurate diagnostic tool for HCC, it seems to be a possible prognostic marker. However, its

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