SAB4200606

Anti-EB3 antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

• 동의어(들): Anti-EBF3, Anti-EBF3-S, Anti-MAPRE3, Anti-RP/EB family, Anti-RP3, Anti-member 3, Anti-microtubule-associated protein
• UNSPSC 코드: 12352203
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• Quality Level: 200
• 결합: unconjugated
• 항체 형태: affinity isolated antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 형태: buffered aqueous solution
• 분자량: antigen ~32 kDa
• 종 반응성: mouse, human, rat
• 농도: ~1.0 mg/mL
• 기술: immunoblotting: 1-2 μg/mL using lysates of rat brain (S1 fraction); immunoprecipitation (IP): 5-10 μg using lysates of mouse brain (S1 fraction).
• UniProt 수납 번호: Q9UPY8
• 배송 상태: dry ice
• 저장 온도: −20°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... MAPRE3(22924)

설명

일반 설명

End-binding protein-3 (EB3), also known as microtubule-associated protein RP/EB 3 (MAPRE3), belongs to a distinct family of “plus-end tracking proteins” (+TIPs). EB proteins are among the most conserved components of the microtubules cytoskeleton. EB proteins contain an N-terminal calponin homology (CH) domain and a unique EB-like C-terminal motif. EB3 is exclusively expressed in the central nervous system and it interacts with a CNS-specific APC homolog APCL.

면역원

Synthetic peptide corresponding to an internal sequence of human EB3, conjugated to KLH. The corresponding sequence is highly conserved (single amino acid substitution) in rat and mouse EB3.

애플리케이션

Anti-EB3 antibody produced in rabbit has been used in:

• immunoblotting
• co-immunoprecipitation
• immunocytochemistry
• glutathione S-transferase (GST) pull-down assay

생화학적/생리학적 작용

In neurons, End-binding protein-3 (EB3) has been shown to interact with scaffold protein ankyrin-G and PSD95 and is required for stabilizing neuronal polarity in mature neurons and in determining neuronal dendritic morphology. EB3 is also involved in MT polymerization and cell migration. EB3 may be involved in stabilizing MTs and anchoring them to centrosomes during spindle formation. Knockout of EB3 inhibits myoblasts elongation and fusion into myotubes.

물리적 형태

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable