SAB4200598

Monoclonal Anti-FTH1 antibody produced in mouse

~1.0 mg/mL, clone FTH24, purified immunoglobulin

• 동의어(들): Monoclonal Anti-FHC, Monoclonal Anti-FTH, Monoclonal Anti-FTH1, Monoclonal Anti-FTHL6, Monoclonal Anti-OK/SW-cl.84, Monoclonal Anti-PIG15, Monoclonal Anti-PLIF, Monoclonal Anti-cell proliferation-inducing gene 15 protein, Monoclonal Anti-ferritin H subunit, Monoclonal Anti-ferritin heavy chain, Monoclonal Anti-ferritin, heavy polypeptide 1, Monoclonal Anti-placenta immunoregulatory factor, Monoclonal Anti-proliferation-inducing protein 15
• UNSPSC 코드: 12352203
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 200
• 결합: unconjugated
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: FTH24, monoclonal
• 형태: buffered aqueous solution
• 종 반응성: human
• 농도: ~1.0 mg/mL
• 기술: flow cytometry: 10-20 μg/test using HeLa cells; immunoblotting: 0.5-1.0 μg/mL using total cell extracts of HEK-293T cells overexpressing FTH1 (expected: ~26kDa).; indirect immunofluorescence: 5-10 μg/mL using HeLa cells
• 동형: IgG1
• UniProt 수납 번호: P02794
• 배송 상태: dry ice
• 저장 온도: −20°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... FTH1(2495)

설명

일반 설명

Monoclonal Anti-FTH1 (mouse IgG1 isotype) is derived from the hybridoma FTH24 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to an internal sequence of human FTH1. Ferritin, a highly conserved iron-binding protein, is composed of two subunits, namely a 21 kDa heavy (H) chain and a 19 kDa light (L) chain. Ferritin heavy chain 1 gene is mapped on human chromosome 11q13. It is mainly found in heart and pancreas.

특이성

Monoclonal Anti- FTH1 recognizes human FTH1.

면역원

synthetic peptide corresponding to an internal sequence of human FTH1. The isotype is determined by ELISA using Mouse Monoclonal Antibody Isotyping Reagents (Sigma ISO-2).

애플리케이션

Monoclonal Anti-FTH1 antibody produced in mouse may be used in:

• immunoblotting
• immunocytochemistry
• flow cytometry
• immunofluorescence

생화학적/생리학적 작용

Ferritin heavy chain catalyzes the first step in iron storage, the oxidation of Fe(II) to Fe(III). In addition to iron buffering, heavy chain ferritin also enhances de novo thymidine biosynthesis. Serum ferritin level is the most commonly used marker for determining iron status stored in the body. It is markedly influenced by factors such as inflammation, malnutrition, malignancy, and iron overload disorders. Studies have found that defects in ferritin proteins are also associated with several neurodegenerative diseases. FTH1 can also be used as a possible prognostic marker for breast cancer.

물리적 형태

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

저장 및 안정성

For extended storage, freeze at -20 °C in working aliquots. Repeated freezing and thawing,or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable