SAB4200522

Anti-IDH1 (C-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

• 동의어(들): Anti-IDP, Anti-PICD, Anti-isocitrate dehydrogenase 1 (NADP+), soluble, IDH
• UNSPSC 코드: 12352203
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• Quality Level: 200
• 결합: unconjugated
• 항체 형태: affinity isolated antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 형태: buffered aqueous solution
• 분자량: antigen ~45 kDa
• 종 반응성: rat, mouse, human
• 농도: ~1.0 mg/mL
• 기술: immunohistochemistry: 20 μg/mL using human kidney.; indirect immunofluorescence: 1-2 μg/mL using SHSY5Y cells.; western blot: 1-2 μg/mL using extracts of NIH3T3 cells and of rat cerebellum (S1 fraction).
• UniProt 수납 번호: O75874
• 배송 상태: dry ice
• 저장 온도: −20°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... IDH1(3417) ; mouse ... Idh1(15926) ; rat ... Idh1(24479)

설명

일반 설명

IDH1 is a cytoplasmic nicotinamide adenine dinucleotide phosphate (NADP⁺)-dependent enzyme, localized both in the cytoplasm and peroxisomes. The IDH1 gene is mapped to human chromosome 2q34. It comprises a tripeptide peroxisome targeting signal 1 in the C-terminal region and is majorly expressed in the liver.

특이성

Anti-IDH1 (C-terminal)specifically recognizes human, rat and mouse IDH1.

면역원

synthetic peptide corresponding to a C-terminal sequence of human IDH1, conjugated to KLH. The corresponding sequence is highly conserved (single amino acid substitution) in rat and mouse IDH1.

애플리케이션

Anti-IDH1 (C-terminal) antibody produced in rabbit may be used in:

• immunoblotting
• immunofluorescence
• immunohistochemistry

생화학적/생리학적 작용

Isocitrate dehydrogenase (IDH) is a key metabolic enzyme that catalyzes the oxidative decarboxylation of isocitrate into α-ketoglutarate (αKG) utilizing either nicotinamide adenine dinucleotide (NAD⁺) or nicotinamide adenine dinucleotide phosphate (NADP⁺) as co-substrates. Although the function and structure of IDH1 have been well characterized, it has only recently been implicated in cancer. IDH1 appears to have a tumor suppressor activity and its inactivation contributes to tumorigenesis partially mediated by induction of the hypoxia-inducible factor 1α (HIF1) pathway. A genome-wide mutation study has shown that IDH1 is mutated in glioblastoma, acute myeloid leukemia (AML) and chondrosarcoma. Mutations in IDH1 specific to Arg132 (R132) impart the enzyme the ability to generate 2-hydroxyglutarate (2HG) instead of αKG. Elevated levels of 2HG are correlated with increased risk of malignant brain tumors, and block cell differentiation by impairing histone demethylation. Several IDH1 mutations have been identified in gliomas, including R132H, R132C, R132S, R132G and R132L, each may result in different tumor type with varied malignant progression.

물리적 형태

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

저장 및 안정성

For continuous use, storeat 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

면책조항

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable