SAB4200461

Anti-Arc (N-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

• 동의어(들): Anti-Activity-regulated cytoskeleton-associated protein, Anti-Arc3.1, Anti-Arg3.1
• UNSPSC 코드: 12352203
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• 결합: unconjugated
• 항체 형태: affinity isolated antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 양식: buffered aqueous solution
• 분자량: antigen ~55 kDa
• 종 반응성: rat, human
• 향상된 검증: recombinant expression; Learn more about Antibody Enhanced Validation
• 농도: ~1.0 mg/mL
• 기술: immunohistochemistry: 20-30 μg/mL using formalin-fixed, paraffin-embedded rat brain.; immunoprecipitation (IP): 2-4 μg using lysates of HEK-293T cells over-expressing human Arc.; western blot: 1.5-3.0 μg/mL using extracts of HEK-293T cells over-expressing human Arc.
• UniProt 수납 번호: Q7LC44
• 배송 상태: dry ice
• 저장 온도: −20°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... ARC(23237) ; rat ... Arc(54323)

설명

일반 설명

Activity-regulated cytoskeleton-associated protein (Arc) is a growth factor and immediate-early gene (IEG) that is enriched in the brain. Arc is located in the distal dendrites and spine. The Arc gene is located on the human chromosome at 8q24.3.

특이성

Anti-Arc(N-terminal) specifically recognizes humanand rat Arc.

면역원

synthetic peptide corresponding to a sequence at the N-terminal region of human Arc, conjugated to KLH. The corresponding sequence is highly conserved (90% sequence identity) in rat and mouse Arc.

애플리케이션

Anti-Arc (N-terminal) antibody produced in rabbit may be used in:

• immunoblotting
• immunoprecipitation
• immunohistochemistry

생화학적/생리학적 작용

Activity-regulated cytoskeleton-associated protein (Arc) induction is required for late long-term potentiation (LTP) and memory consolidation. It also plays a role in LTP-induced cofilin phosphorylation and F-actin stabilization. This protein affects synaptic plasticity. Arc regulates the endocytosis of α-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) receptors through its interaction with endocytic proteins endophilin2/3 and dynamin. It is associated with notch signaling and spine morphology.

물리적 형태

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

저장 및 안정성

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution bycentrifugation before use. Working dilutions should be discarded if not used within 12 hours.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable