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Merck
모든 사진(5)

주요 문서

SAB4200394

Sigma-Aldrich

Anti-Nestin (C-terminal) antibody produced in rabbit

enhanced validation

~1.5 mg/mL, affinity isolated antibody

동의어(들):

Anti-NES

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About This Item

UNSPSC 코드:
12352203
NACRES:
NA.41

생물학적 소스

rabbit

Quality Level

결합

unconjugated

항체 형태

affinity isolated antibody

항체 생산 유형

primary antibodies

클론

polyclonal

형태

buffered aqueous solution

분자량

antigen ~300 kDa

종 반응성

mouse, rat

포장

antibody small pack of 25 μL

향상된 검증

recombinant expression
Learn more about Antibody Enhanced Validation

농도

~1.5 mg/mL

기술

indirect immunofluorescence: 1-2 μg/mL using mouse P19 cells
western blot: 2-4 μg/mL using lysates of HEK293T cells over-expressing rat nestin.

UniProt 수납 번호

배송 상태

dry ice

저장 온도

−20°C

타겟 번역 후 변형

unmodified

유전자 정보

mouse ... Nes(18008)
rat ... Nes(25491)

일반 설명

Nestin/NES belongs to the intermediate filament (IF) protein family. Its expression is found in immature neuroepithelial and progenitor cell types. This 240 kDa protein contains the shortest head domain (N-terminus) and a long tail domain (C-terminus). Nestin is mapped to human chromosome 1q23.1.

면역원

synthetic peptide corresponding to a sequence at the C- terminus of mouse nestin, conjugated to KLH. The sequence is identical in rat nestin.

애플리케이션

Anti-Nestin (C-terminal) antibody has been used in immunostaining.

생화학적/생리학적 작용

Nestin/NES plays a major role in the modulation of assembly and disassembly of intermediate filaments, which in turn help in the remodeling of the cell along with other structural proteins.

물리적 형태

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Nestin-expressing cell types in the temporal lobe and hippocampus: Morphology, differentiation, and proliferative capacity.
Liu J, et al.
Glia, 66(1), 62-77 (2018)
Nestin structure and predicted function in cellular cytoskeletal organisation.
Michalczyk K and Ziman M
Histology and Histopathology, 20(2), 665-671 (2005)
YAP stabilizes SMAD1 and promotes BMP2-induced neocortical astrocytic differentiation.
Huang Z, et al.
Development, 143(13), 2398-2409 (2016)
Nestin expression in embryonic human neuroepithelium and in human neuroepithelial tumor cells.
Tohyama T, et al.
Laboratory Investigation; a Journal of Technical Methods and Pathology, 66(3), 303-313 (1992)
Intermediate filaments in peripheral nervous system: Their expression, dysfunction and diseases
Parlakian A, et al.
Revista de Neurologia, 172(10), 607-613 (2016)

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