SAB4200256

Anti-AKT1S1 antibody, Mouse monoclonal

clone AKT1S1-3, purified from hybridoma cell culture

• 동의어(들): Monoclonal Anti-AKT1 substrate 1 (proline-rich), Monoclonal Anti-Lobe, Monoclonal Anti-PRAS40, Monoclonal Anti-PROLINE-RICH AKT SUBSTRATE, 40-KD
• UNSPSC 코드: 12352203
• NACRES: NA.44

속성

• 생물학적 소스: mouse
• Quality Level: 200
• 결합: unconjugated
• 항체 형태: purified from hybridoma cell culture
• 항체 생산 유형: primary antibodies
• 클론: AKT1S1-3, monoclonal
• 양식: buffered aqueous solution
• 분자량: antigen ~40 kDa
• 종 반응성: human
• 농도: ~1.0 mg/mL
• 기술: immunoprecipitation (IP): suitable; western blot: 2-8 μg/mL using whole extracts of human MDA-MB-231cells.
• 동형: IgG1
• UniProt 수납 번호: Q96B36
• 배송 상태: dry ice
• 저장 온도: −20°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... AKT1S1(84335)

설명

일반 설명

AKT1S1 is a raptor-associated protein that interacts with mTORC1 and subsequently inhibits the mTORC1 signaling pathway. This raptor-binding protein also inhibits phosphorylation of S6K1 and cell growth. Expression of AKT1S1 has been implicated in breast and pulmonary carcinogenesis, as well as in mediating insulin sensitivity . Monoclonal Anti-AKT1S1 antibody is specific for human AKT1S1 (approx. 40 kDa).

Monoclonal Anti-AKT1S1 (mouse IgG1 isotype) is derived from the hybridoma AKT1S1-3 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a fusion protein corresponding to the N-terminus of human AKT1S1. Akt1 substrate 1 (AKT1S1) is also named as proline-rich Akt-substrate of 40 kDa (PRAS40). The rapamycin-sensitive mammalian target of rapamycin complex 1 (mTORC1) consists of mTOR, raptor, mLST8 and AKT1S1, that controls protein translation. AKT1S1 contains a TOR signaling (TOS) motif that mediates its binding to mTORC1.

면역원

The corresponding sequence differs by a single amino acid in rat, and by 2 amino acids in mouse AKT1S1.

fusion protein corresponding to the N-terminus of human AKT1S1.

애플리케이션

Monoclonal Anti-AKT1S1 antibody is suitable for use in western blot (2-8 μg/mL using whole extracts of human MDA-MB-231cells) and immunoprecipitation.

생화학적/생리학적 작용

Akt1 substrate 1 (AKT1S1) is involved in the PI3KAkt/ protein kinase B (PKB) survival pathway. Phosphorylation of AKT1S1 by Akt and mammalian target of rapamycin complex 1 (mTORC1) disrupts the binding between mTORC1 and AKT1S1, relieves the inhibitory effect of AKT1S1 on mTORC1 activity, and leads to the binding of AKT1S1 to 14-3-3, a cytosolic anchor protein. The binding of AKT1S1 to 14-3-3 requires amino acids and insulin, which is partially inhibited by rapamycin.

물리적 형태

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable