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Merck
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Key Documents

S8140

Sigma-Aldrich

SSPE Buffer

for Northern and Southern blotting, powder blend

동의어(들):

Saline-Sodium Phosphate-EDTA Buffer

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About This Item

UNSPSC 코드:
41105319
NACRES:
NA.25

Grade

for molecular biology

Quality Level

형태

powder blend

pH

7.2-7.6 (20 × in water, high purity, ambient)

solubility

water, high purity: 20 ×, clear, colorless

외래 활성

DNAse, none detected
Endonuclease, none detected
Exonuclease, none detected
NICKase, none detected
RNAse, none detected

저장 온도

room temp

일반 설명

SSPE Buffer 20x powder is a standard reagent in Southern and Northern hybridization procedures. This powder should be reconstituted to the indicated volume with molecular biology grade water before use. The resulting 20x liquid can be used straight or diluted as needed.

애플리케이션


  • Influence of Saline Buffers over the Stability of High-Annealed Gold Nanoparticles Formed on Coverslips for Biological and Chemosensing Applications.: This study explores the role of saline buffers in maintaining the stability of high-annealed gold nanoparticles used in biological and chemosensing applications. The findings highlight the potential for enhanced sensor performance in detecting biological molecules (Zhou & Ionescu, 2020).

  • Quantification of Surface Etching by Common Buffers and Implications on the Accuracy of Label-Free Biological Assays.: This research assesses the impact of various buffers, including SSPE buffer, on the surface etching of substrates used in label-free biological assays. The results indicate that buffer-induced surface changes can significantly affect assay accuracy (Ahn et al., 2012).

특징 및 장점

  • Easy to reconstitute powder
  • Multi-purpose use in hybridizations

성분

Final SSPE Buffer 20x contains 0.02M EDTA and 2.98M NaCl in 0.2M phosphate buffer (pH 7.4).

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges


시험 성적서(COA)

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