S2657

Anti-S100B Antibody

mouse monoclonal, SH-B4

• 동의어(들): Anti-NEF, Anti-S100, Anti-S100-B, Anti-S100beta
• MDL number: MFCD00162834
• UNSPSC 코드: 12352203
• NACRES: NA.41

속성

• 제품명: Monoclonal Anti-S-100 (β-Subunit) antibody produced in mouse, clone SH-B4, (Solution in 0.01M phosphate buffered saline pH 7.4, containing 15mM sodium azide as a preservative)
• 생물학적 소스: mouse
• 결합: unconjugated
• 항체 형태: (Solution in 0.01M phosphate buffered saline pH 7.4, containing 15mM sodium azide as a preservative)
• 항체 생산 유형: primary antibodies
• 클론: SH-B4, monoclonal
• 포함: 15 mM sodium azide
• 종 반응성: sheep, bovine, goat, pig, feline, rabbit, rat, canine, human
• 기술: immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable (: suitable); indirect ELISA: suitable (:suitable); western blot: 1:100-1:200 ( (using S-100B Protein, Bovine Brain))
• 동형: IgG1
• UniProt 수납 번호: P04271
• 배송 상태: dry ice
• 저장 온도: −20°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... S100B(6285) ; rat ... S100b(25742)

설명

일반 설명

Monoclonal Anti-S-100 (b-Subunit) (mouse IgG1 isotype) is derived from the SH-B4 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice. S-100 is a set of small, highly acidic, thermolabile dimer proteins of ~20 kDa, widely distributed in different tissues.

S-100 (β-Subunit) is a calcium-binding protein, that belongs to S100 family of calcium-binding proteins. It is expressed in melanocytes. It is located on human chromosome 21q22.3.

특이성

Recognizes an epitope located on the β chain (i.e. in S-100a and S-100b) but not on the α chain of S-100 (i.e. in S-100a and S-100ao). In ELISA, recognition of S-100 subunit by clone SH-B4 is Ca²⁺-dependent. The product does not react with other members of the EF-hand family such as, calmodulin, parvalbumin, intestinal calcium-binding protein and myosin light chain. In immunohistochemistry, the antibody detects normal and neoplastic S-100 β subunit-containing cells (e.g. Schwann cells, chondrocytes, melanocytes, and melanotic tumors) in protease-digested, formalin-fixed, paraffin-embedded tissues.

면역원

bovine brain S-100b

애플리케이션

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunocytochemistry (1 paper)

Monoclonal Anti-S-100 (β-Subunit) antibody produced in mouse has been used in immunocytochemistry and immunohistochemistry. It has also been used as a component in serum medium for 5-ethynyl-29-deoxyuridine (EdU), incorporation assay.

생화학적/생리학적 작용

S-100 (β-Subunit) acts as a marker of melanocyte cytotoxicity. Reduced expression of S100B is observed in chronic liver diseases. It also acts as a serum marker in endocrine resistant breast cancer.

S-100 is considered to be a cell-growth regulator, but other functions have been suggested, e.g., increasing the membrane permeability to cations under physiologic conditions, stimulation of nucleolar RNA polymerase activity, interaction with the tumor suppressor protein p53 and as a carrier of proteins and free fatty acids in adipocytes.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable