R9778

Anti-hnRNP-A1 antibody, Mouse monoclonal

~2 mg/mL, clone 4B10, purified from hybridoma cell culture

• 동의어(들): Anti-Heterogeneous Nuclear Ribonucleoprotein-A1
• MDL number: MFCD06798702
• UNSPSC 코드: 12352203
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 200
• 결합: unconjugated
• 항체 형태: purified from hybridoma cell culture
• 항체 생산 유형: primary antibodies
• 클론: 4B10, monoclonal
• 형태: buffered aqueous solution
• 분자량: antigen 32-35 kDa
• 종 반응성: bovine, human, canine
• 농도: ~2 mg/mL
• 기술: immunocytochemistry: suitable; immunoprecipitation (IP): suitable; indirect ELISA: suitable; microarray: suitable; western blot: 0.25-0.5 μg/mL using total cell extract of HeLa cells
• 동형: IgG2a
• UniProt 수납 번호: P09651
• 배송 상태: dry ice
• 저장 온도: −20°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... HNRNPA1(3178)

설명

일반 설명

Monoclonal Anti-hnRNP-A1 (mouse IgG2a isotype) is derived from the 4B10 hybridoma produced by the fusion of mouse myeloma cells (SP2/0 cells) and splenocytes from NZB mice immunized with purified human hnRNPA1. Heterogeneous nuclear ribonucleoprotein A1 (hnRNPs) consist of protein groups named A to U and many of these protein groups consist of more than one isoform. The major steady-state proteins of the isolated hnRNP complex are A1, A2, B1, B2, C1, and C2, with a range of molecular weight starting with 34 kDa up to 43 kDa. hnRNP-A1 is ubiquitously expressed in cells and tissues.

면역원

purified human hnRNP-A1.

애플리케이션

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Monoclonal Anti-hnRNP-A1 antibody produced in mouse has also been used in:

• enzyme linked immunosorbent assay (ELISA)
• immunoblotting
• immunoprecipitation
• immunocytochemistry.

생화학적/생리학적 작용

Heterogeneous nuclear ribonucleoprotein A1 (hnRNP-A1) is important in biological activities such as transcription, pre-mRNA processing, cytoplasmic mRNA translation and turnover. hnRNP-A1 is important in pre-mRNA processing and in mRNA export from the nucleus. The protein contains a 38-amino acid domain called M9, which is important for the interaction with the transportin protein and therefore, for its import and export from the nucleus. RanGTP mediates dissociation of hnRNP-A1 from transportin. Higher expression is observed in proliferating and/or transformed cells than in differentiated tissues.

물리적 형태

Solution in 0.01 M phosohate buffered saline, pH 7.4, and 15 mM sodium azide.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)